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anti-Rat (Rattus) RPS6KB1 antibody for Immunofluorescence

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • p70S6K
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • ribosomal protein S6 kinase, polypeptide 1
  • RPS6KB1
  • Rps6kb1
Epitope
pThr389
101
32
27
26
24
24
20
20
19
18
18
18
18
16
15
15
15
14
14
10
9
8
7
6
6
6
5
5
5
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
666
443
420
73
53
41
34
33
21
13
13
8
5
5
3
3
1
Host
Rabbit
594
52
23
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
14
13
13
9
9
9
9
8
8
8
8
8
8
7
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
511
264
243
139
118
102
64
36
21
20
17
11
8
1
1
1
1
Options
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Catalog No. ABIN1531360
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.099935 ABIN968442 IF WB Mouse IgG1 AA 2-121 Log in to see 16-p70[s6k] 4
11.099935 ABIN968441 IF WB Mouse IgG1 AA 2-121 Log in to see 16-p70[s6k] 4
11.099935 ABIN1531361 IF IHC ELISA WB Rabbit IgG pSer418 Log in to see Polyclonal
11.099935 ABIN1532376 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
11.099935 ABIN1532377 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
11.099935 ABIN1870601 IF IHC WB Rabbit IgG pThr421 Log in to see Polyclonal
11.099935 ABIN2705221 IC IF IHC WB Rabbit C-Term, pSer447 Log in to see Polyclonal
11.099935 ABIN2705213 IC IF IHC WB Rabbit C-Term, pSer418 Log in to see Polyclonal
11.099935 ABIN2705217 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.099935 ABIN2705218 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.099935 ABIN2707004 IC IF IHC WB Rabbit Center Log in to see Polyclonal
11.099935 ABIN2705220 IC IF IHC WB Rabbit C-Term, pSer444 Log in to see Polyclonal
11.099935 ABIN2705219 IC IF IP IHC WB Rabbit C-Term, pSer434 Log in to see Polyclonal
11.099935 ABIN1870599 IF IHC WB Rabbit IgG pSer424 Log in to see Polyclonal
11.099935 ABIN1870597 IF IHC WB Rabbit IgG pSer411 Log in to see Polyclonal
11.099935 ABIN2801328 IC IF IHC WB Rabbit Log in to see Polyclonal
11.099935 ABIN2425025 IF IHC WB Rabbit IgG pSer411 Log in to see Polyclonal
11.099935 ABIN2425039 IF IHC WB Rabbit IgG pSer424 Log in to see Polyclonal
1 ABIN361700 ICC IF IP ELISA WB Rabbit C-Term Log in to see Polyclonal 6
1 ABIN197014 IF IHC (p) WB Rabbit pThr421 Log in to see Polyclonal 2

Top referenced anti-RPS6KB1 antibody for Immunofluorescence

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 110 Antibodies 118 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 103 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 102 Antibodies
Immunohistochemistry (IHC) 156 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 82 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 292 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope pThr389
(101), (32), (27), (26), (24), (24), (20), (20), (19), (18), (18), (18), (18), (16), (15), (15), (15), (14), (14), (10), (9), (8), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(666), (443), (420), (73), (53), (41), (34), (33), (21), (13), (13), (8), (5), (5), (3), (3), (1)
Host Rabbit
(594), (52), (23), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (14), (13), (13), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(511), (264), (243), (139), (118), (102), (64), (36), (21), (20), (17), (11), (8), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN1531360) Images
Specificity P70 S6 Kinase (Phospho-Thr389) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Thr389.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr389.
Isotype IgG

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 Antibody (ABIN1531360) Images back to top
Antigen
Alternative Name p70 S6 Kinase (RPS6KB1 Antibody Abstract)
Background Molecular weight of target protein: 59 kDa
Synonyms: Ribosomal protein S6 kinase I, S6K, S6K1, 70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase alpha, p70(S6K)-alpha, p70-S6K, P70S6K, p70-alpha
Molecular Weight 59 kDa
Gene ID 6198
OMIM 608938
UniProt P23443
Research Area Signaling, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Smooth Muscle Cell Migration

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 Antibody (ABIN1531360) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.463642 (NCBI Gene Symbol: RPS6KB1)

Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 Antibody (ABIN1531360) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-RPS6KB1 Antibody (ABIN1531360)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Product cited in:

Chan, Wang, Fang, Chen: "Fibrous papule of the face, similar to tuberous sclerosis complex-associated angiofibroma, shows activation of the mammalian target of rapamycin pathway: evidence for a novel therapeutic strategy?" in: PLoS ONE, Vol. 9, Issue 2, pp. e89467, 2014

Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN1531360) back to top
Supplier Images
Western Blotting (WB) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (pThr389) (ABIN1531360) Western blot analysis of extracts from Jurkat cells treated with Insulin 0.01U/ml 15'...
Immunohistochemistry (IHC) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (pThr389) (ABIN1531360) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 ...
Immunofluorescence (IF) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (pThr389) (ABIN1531360) Immunofluorescence analysis of HuvEc cells, using p70 S6 Kinase (Phospho-Thr389) Anti...