anti-Rat (Rattus) RPS6KB1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • XPKAbeta
  • pkacb
  • 10539
  • 7084
  • CG10539
  • DS6K
  • Dmel\\CG10539
  • Dp70S6k
  • Dp70[s6k]
  • Dp70s6k
  • S6k
  • dS6K
  • dS6k
  • dp70[S6k]
  • dp70s6k
  • dps6k
  • ds6k
  • fs(3)07084
  • l(3)07084
  • p70 S6K
  • p70/S6K
  • p70S6K
  • p70[S6 kinase]
  • p70[S6K]
  • p70[S6k]
  • p70[S6kinase]
  • p70s6K
  • s6k
  • s6k11
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • p70s6k-A
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • protein kinase, cAMP-dependent, catalytic, beta
  • RPS6-p70-protein kinase
  • ribosomal protein S6 kinase, polypeptide 1
  • p70 S6 kinase
  • RPS6KB1
  • prkacb
  • S6k
  • Rps6kb1
  • p70s6k-A
Epitope
pSer417
110
41
39
30
30
20
20
20
19
18
18
17
15
15
14
14
14
14
13
11
10
9
8
6
6
6
5
5
5
5
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
709
445
422
73
54
41
34
33
21
13
13
8
5
5
3
3
1
Host
Rabbit
636
53
23
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
14
13
13
9
9
9
9
9
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
546
282
257
152
132
103
67
42
22
20
17
11
7
2
1
1
1
1
Options
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Catalog No. ABIN712976
$ 317.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN873120 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN713006 IF (p) IHC (p) WB Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
1 ABIN686377 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN713051 IF (p) IHC (p) WB Rabbit IgG pSer441, pThr444 Log in to see Polyclonal
1 ABIN2814986 IF (p) Alexa Fluor 594 Rabbit IgG AA 300-350 Log in to see Polyclonal
1 ABIN712991 IF (p) IHC (p) WB Rabbit IgG pThr412 Log in to see Polyclonal
1 ABIN2814987 IF (p) Alexa Fluor 594 Rabbit IgG AA 412-419, pSer417 Log in to see Polyclonal
1 ABIN2814974 IF (p) Alexa Fluor 594 Rabbit IgG AA 280-330 Log in to see Polyclonal
1 ABIN2814988 IF (p) Alexa Fluor 594 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
1 ABIN712981 IF (p) Cy5.5 Rabbit IgG pSer417 Log in to see Polyclonal
1 ABIN712980 IF (p) Cy5 Rabbit IgG pSer417 Log in to see Polyclonal
1 ABIN712982 IF (p) Cy7 Rabbit IgG pSer417 Log in to see Polyclonal
1 ABIN713009 IF (p) Cy3 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
1 ABIN713010 IF (p) Cy5 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
1 ABIN713013 IF (p) FITC Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
1 ABIN712983 IF (p) FITC Rabbit IgG pSer417 Log in to see Polyclonal
1 ABIN713012 IF (p) Cy7 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
1 ABIN712979 IF (p) Cy3 Rabbit IgG pSer417 Log in to see Polyclonal
1 ABIN713011 IF (p) Cy5.5 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
1 ABIN686381 IF (p) Cy5 Rabbit IgG Log in to see Polyclonal

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope pSer417
(110), (41), (39), (30), (30), (20), (20), (20), (19), (18), (18), (17), (15), (15), (14), (14), (14), (14), (13), (11), (10), (9), (8), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(709), (445), (422), (73), (54), (41), (34), (33), (21), (13), (13), (8), (5), (5), (3), (3), (1)
Host Rabbit
(636), (53), (23), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (14), (13), (13), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(546), (282), (257), (152), (132), (103), (67), (42), (22), (20), (17), (11), (7), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN712976) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human RPS6KB1 around the phosphorylation site of Ser417
Isotype IgG

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN712976) Images back to top
Antigen
Alternative Name Rps6kb1 (RPS6KB1 Antibody Abstract)
Background

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B.

Subcellular location: Cytoplasm, Nucleus

Synonyms: S6K, PS6K, S6K1, STK14A, p70-S6K, p70 S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, RPS6KB1

Gene ID 6198
UniProt P23443
Research Area Signaling, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN712976) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details ProductDetails: References for anti-RPS6KB1 antibody (ABIN712976) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

ProductDetails: References for anti-RPS6KB1 antibody (ABIN712976)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Product cited in:

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014