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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||17 Antibodies||15 Antibodies||15 Antibodies|
|Simple Western (SimWes)||1 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||503 Antibodies||313 Antibodies||292 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies||1 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||63 Antibodies||43 Antibodies||44 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||116 Antibodies|
|Antigen||Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RPS6KB1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-RPS6KB1 AntibodyTarget Details RPS6KB1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p70 S6K protein. (region surrounding Pro412)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details RPS6KB1Product Details anti-RPS6KB1 Antibody Application Details Handling Images back to top
|Alternative Name||RPS6KB1 / STK14A (RPS6KB1 Antibody Abstract)|
|Background||P70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal. Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade. The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains.Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1|
|Molecular Weight||approx. 59,70,85 kDa|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Smooth Muscle Cell Migration|
Application DetailsProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling Images back to top
ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.09 % sodium azide, 50 % glycerol|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling back to top