Human Transportin 1 ELISA Kit

Recommended Transportin 1 Product (supplied by: Log in to see )

Antigen
Transportin 1 (TNPO1) ELISA Kits
  • TNPO1
  • ATTRN1
  • F12A24.13
  • F12A24_13
  • TRANSPORTIN 1
  • transportin 1
  • DKFZp469O204
  • mip1
  • zgc:100858
  • JC310
  • MIP1
  • SIN1
  • SIN1b
  • SIN1g
  • IPO2
  • KPNB2
  • MIP
  • TRN
  • AU021749
  • D13Ertd688e
  • Kpnb2
  • Mip1
  • Znf667
  • transportin 1
  • major intrinsic protein of lens fiber a
  • mitogen-activated protein kinase associated protein 1
  • zinc finger protein 667
  • TNPO1
  • tnpo1
  • TRN1
  • mipa
  • MAPKAP1
  • Tnpo1
  • Zfp667
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
7
6
2
2
2
2
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
3.12-200 ng/mL
Minimum Detection Limit
3.12 ng/mL
Application
ELISA
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Catalog No. ABIN1572842
$ 908.41
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.865132 ABIN1117590 Sandwich ELISA Serum, Plasma, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN511054 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 5.0-100 ng/mL 5.0 ng/mL Log in to see
1 ABIN5525993 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN4973513 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN2952025 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN5071969 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

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General

Antigen Transportin 1 (TNPO1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(7), (6), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 3.12-200 ng/mL
Minimum Detection Limit 3.12 ng/mL
Application ELISA
Supplier Log in to see

Product Details Transportin 1 ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TNPO1 in human serum, plasma, tissue homogenates and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 1.06 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Target details

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Antigen
Alternative Name TNPO1 (TNPO1 ELISA Kit Abstract)
Gene ID 3842
UniProt Q92973
Pathways PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process, Protein targeting to Nucleus, CXCR4-mediated Signaling Events

Application Details

Product Details Transportin 1 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Assay Procedure The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Handling

Product Details Transportin 1 ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Expiry Date 12 months

Images

Product Details Transportin 1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Transportin 1 ELISA Kit (TNPO1) (ABIN1572842) Transportin 1 (TNPO1) ELISA Kit