RPSG2 Protein (AA 2-179, full length) (His tag)

Catalog No. ABIN1046353

Product Details

Target: RPSG2 (30S Ribosomal Protein S7 (RPSG2))

Protein Type: Recombinant

Protein Characteristics: AA 2-179, full length

Origin: E. coli

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RPSG2 protein is labelled with His tag.

Application: ELISA, SDS-PAGE (SDS)

Sequence: PRRRVIGQRK ILPDPKFGSE LLAKFVNILM VDGKKSTAES IVYSALETLA QRSGKSELEA FEVALENVRP TVEVKSRRVG GSTYQVPVEV RPVRRNALAM RWIVEAARKR GDKSMALRLA NELSDAAENK GTAVKKREDV HRMAEANKAF AHYRWLSLRS FSHQAGASSK QPALGYLN

Characteristics: One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites, it probably blocks exit of the E site tRNA. Ref.11 Protein S7 is also a translational repressor protein, it regulates the expression of the str operon members to different degrees by binding to its mRNA. Ref.11

Purity: 90 %

Application Details

Comment:

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20mM Tris-Hcl, 0.5M NaCl, 10% glycerin, PH 8.0, 200 mM Imidazole

Storage: -20 °C

References

Blattner, Plunkett, Bloch, Perna, Burland, Riley, Collado-Vides, Glasner, Rode, Mayhew, Gregor, Davis, Kirkpatrick, Goeden, Rose, Mau, Shao: "The complete genome sequence of Escherichia coli K-12." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1453-62, (1997) (PubMed).

Timms, Steingrimsdottir, Lehmann, Bridges: "Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesis." in: Molecular & general genetics : MGG, Vol. 232, Issue 1, pp. 89-96, (1992) (PubMed).

Post, Nomura: "DNA sequences from the str operon of Escherichia coli." in: The Journal of biological chemistry, Vol. 255, Issue 10, pp. 4660-6, (1980) (PubMed).

Target Details

Target: RPSG2 (30S Ribosomal Protein S7 (RPSG2))

Alternative Name: 30S Ribosomal Protein S7 (RPSG2 Products)

Molecular Weight: 24 kD

UniProt: P02359