CKMT1 Protein (AA 40-417, full length) (His tag)

Catalog No. ABIN1046871

Product Details

Target: CKMT1 (Creatine Kinase, Mitochondrial 1, Ubiquitous (CKMT1))

Protein Type: Recombinant

Protein Characteristics: AA 40-417, full length

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CKMT1 protein is labelled with His tag.

Application: ELISA

Sequence: ASERRRLYPP SAEYPDLRKH NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH PFIKTVGMVA GDEETYEVFA DLFDPVIQER HNGYDPRTMK HTTDLDASKI RSGYFDERYV LSSRVRTGRS IRGLSLPPAC TRAERREVER VVVDALSGLK GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG MARDWPDARG IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE RGWEFMWNER LGYILTCPSN LGTGLRAGVH IKLPLLSKDS RFPKILENLR LQKRGTGGVD TAATGGVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR LERGQDIRIP TPVIHTKH

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, (2003) (PubMed).

Haas, Korenfeld, Zhang, Perryman, Roman, Strauss: "Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase." in: The Journal of biological chemistry, Vol. 264, Issue 5, pp. 2890-7, (1989) (PubMed).

Target Details

Target: CKMT1 (Creatine Kinase, Mitochondrial 1, Ubiquitous (CKMT1))

Alternative Name: Creatine Kinase U-Type, Mitochondrial Protein (CKMT1 Products)

Synonyms: fb37h03 Protein, fb69a12 Protein, wu:fb37h03 Protein, wu:fb69a12 Protein, Mt-CK Protein, ScCKmit Protein, UbCKmit Protein, mi-CK Protein, Ckmt1 Protein, creatine kinase, mitochondrial 1 Protein, creatine kinase, mitochondrial 1, ubiquitous Protein, ckmt1 Protein, Ckmt1 Protein

Background: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Molecular Weight: 47.2 kD

UniProt: P12532