TNF alpha Protein (AA 78-234) (His tag)

Catalog No. ABIN1047832

Product Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Protein Characteristics: AA 78-234

Origin: Cow

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TNF alpha protein is labelled with His tag.

Application: SDS-PAGE (SDS), ELISA

Sequence: LRSSSQASSN KPVAHVVADI NSPGQLRWWD SYANALMANG VKLEDNQLVV PADGLYLIYS QVLFRGQGCP STPLFLTHTI SRIAVSYQTK VNILSAIKSP CHRETPEWAE AKPWYEPIYQ GGVFQLEKGD RLSAEINLPD YLDYAESGQV YFGIIAL

Characteristics: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Purity: 95 %

Application Details

Comment:

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20mM Tris-Hcl, 0.5M NaCl, 10% glycerin, PH 8.0, 200 mM Imidazole

Storage: -20 °C

References

Dietz, Neibergs, Womack, Kehrli: "Rapid communication: single strand conformational polymorphism (SSCP) of bovine tumor necrosis factor alpha." in: Journal of animal science, Vol. 75, Issue 9, pp. 2567, (1997) (PubMed).

Mertens, Muriuki, Gaidulis: "Cloning of two members of the TNF-superfamily in cattle: CD40 ligand and tumor necrosis factor alpha." in: Immunogenetics, Vol. 42, Issue 5, pp. 430-1, (1995) (PubMed).

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor necrosis factor (TNF alpha Products)

Synonyms: DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein

Background: Synonyms: Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a

Molecular Weight: 21.5 kD

UniProt: Q06599

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome