UCHL1 Protein (AA 1-222, partial) (His tag)

Catalog No. ABIN1047864

Product Details

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Protein Type: Recombinant

Protein Characteristics: AA 1-222, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UCHL1 protein is labelled with His tag.

Application: ELISA

Sequence: MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KA

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Target Details

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1 protein (UCHL1 Products)

Synonyms: PARK5 Protein, PGP 9.5 Protein, PGP9.5 Protein, PGP95 Protein, Uch-L1 Protein, UCH-L1 Protein, UCHL1 Protein, cb358 Protein, wu:fc55h08 Protein, park5 Protein, pgp9.5 Protein, uch-l1 Protein, MGC132191 Protein, uchl1 Protein, AW822034 Protein, C88048 Protein, R75593 Protein, UCHL-1 Protein, gad Protein, ubiquitin C-terminal hydrolase L1 Protein, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) Protein, ubiquitin C-terminal hydrolase L1 L homeolog Protein, ubiquitin carboxyl-terminal esterase L1 Protein, ubiquitin carboxy-terminal hydrolase L1 Protein, UCHL1 Protein, uchl1 Protein, uchl1.L Protein, LOC100304750 Protein, Uchl1 Protein

Background: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Molecular Weight: 28.8 kD

UniProt: P09936

Pathways: Feeding Behaviour