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Staining protocol: 1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS) 2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently. For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M). 3. Incubate for 15min at room temperature in the dark 4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour).