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CD73 Protein (AA 27-547) (His tag)

NT5E Origin: Human Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Catalog No. ABIN1095954
  • Target See all CD73 (NT5E) Proteins
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 27-547
    Origin
    • 16
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 17
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    Human Cells
    Purification tag / Conjugate
    This CD73 protein is labelled with His tag.
    Purpose
    Recombinant Human 5'-Nucleotidase/5'-NT/CD73 (C-6His)
    Sequence
    WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHA DETFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI KVDHHHHHH
    Characteristics
    Recombinant Human 5'-Nucleotidase/NT5E is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Trp27-Lys547) of Human NT5E fused with a polyhistidine tag at the C-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
    Top Product
    Discover our top product NT5E Protein
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 120 mM NaCl,4 mM CaCl2, 20 % Glycerol, pH 7.5.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Storage
    -80 °C
    Storage Comment
    Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.
    Expiry Date
    6 months
  • Target
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Alternative Name
    cd73 (NT5E Products)
    Synonyms
    CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein
    Sub Type
    Fusionprotein
    Background
    CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
    Alternative Names: 5'-Nucleotidase, 5'-NT, Ecto-5'-Nucleotidase, CD73, NT5E, NT5, NTE
    Molecular Weight
    58.8 kDa
    UniProt
    P21589
    Pathways
    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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