Arylsulfatase A Protein (ARSA) (AA 19-507) (His tag)

Catalog No. ABIN1096050

Product Details

Target: Arylsulfatase A (ARSA)

Protein Type: Recombinant

Protein Characteristics: AA 19-507

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This Arylsulfatase A protein is labelled with His tag.

Purpose: Recombinant Human Arylsulfatase A/ARSA (C-6His)

Sequence: RPPNIVLIFA DDLGYGDLGC YGHPSSTTPN LDQLAAGGLR FTDFYVPVSL CTPSRAALLT GRLPVRMGMY PGVLVPSSRG GLPLEEVTVA EVLAARGYLT GMAGKWHLGV GPEGAFLPPH QGFHRFLGIP YSHDQGPCQN LTCFPPATPC DGGCDQGLVP IPLLANLSVE AQPPWLPGLE ARYMAFAHDL MADAQRQDRP FFLYYASHHT HYPQFSGQSF AERSGRGPFG DSLMELDAAV GTLMTAIGDL GLLEETLVIF TADNGPETMR MSRGGCSGLL RCGKGTTYEG GVREPALAFW PGHIAPGVTH ELASSLDLLP TLAALAGAPL PNVTLDGFDL SPLLLGTGKS PRQSLFFYPS YPDEVRGVFA VRSGKYKAHF FTQGSAHSDT TADPACHASS SLTAHEPPLL YDLSKDPGEN YNLLGGVAGA TPEVLQALKQ LQLLKAQLDA AVTFGPSQVA RGEDPALQIC CHPGCTPRPA CCHCPDPHAV DHHHHHH

Characteristics: Recombinant Human Arylsulfatase A/ARSA is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Arg19-507Ala) of Human ARSA fused with a polyhistidine tag at the C-terminus

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Arylsulfatase A (ARSA)

Alternative Name: arsa (ARSA Products)

Synonyms: ARSA Protein, zgc:101575 Protein, arsa Protein, AS-A Protein, ASA Protein, AW212749 Protein, As-2 Protein, As2 Protein, TISP73 Protein, MLD Protein, mld Protein, arylsulfatase A Protein, arylsulfatase Protein, arylsulfatase A, gene 1 S homeolog Protein, ARSA Protein, arsa Protein, arsA Protein, RB6599 Protein, Arsa Protein, arsa.1.S Protein

Sub Type: Fusionprotein

Background: Arylsulfatase A (ARSA) is a lysosomal enzyme that breaks down Cerebroside 3-Sulfate, a major constituent of the myelin sheet, into Cerebroside and Sulfate. The ARSA deficiency results in Metachromatic Leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms. Recombinant Human ARSA corresponds to the ARSA mature peptide and has sulfatase activity.
Alternative Names: Arylsulfatase A, ASA, Cerebroside-Sulfatase, ARSA

Molecular Weight: 52.9 kDa

UniProt: P15289