ATG10 Protein (AA 1-190) (His tag,T7 tag)
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- Target See all ATG10 Proteins
- ATG10 (Autophagy Related 10 (ATG10))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-190
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ATG10 protein is labelled with His tag,T7 tag.
- Purpose
- Recombinant Human Autophagy Related 10 Homolog/ATG10 (C-6His, N-T7 tag)
- Sequence
- MASMTGGQQM GRGSMEEDEF IGEKTFQRYC AEFIKHSQQI GDSWEWRPSK DCSDGYMCKI HFQIKNGSVM SHLGASTHGQ TCLPMEEAFE LPLDDCEVIE TAAASEVIKY EYHVLYSCSY QVPVLYFRAS FLDGRPLTLK DIWEGVHECY KMRLLQGPWD TITQQEHPIL GQPFFVLHPC KTNEFMTPVL KNSQKINKNV NYITLEHHHH HH
- Characteristics
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Recombinant Human Autophagy Related 10 Homolog/ATG10 (C-6His, N-T7 tag)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product ATG10 Protein
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Autophagy Related 10 (ATG10) (AA 1-220) protein (His tag)
ATG10 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Autophagy Related 10 (ATG10) (AA 1-220) protein (GST tag)ATG10 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Autophagy Related 10 (ATG10) (AA 1-220) protein (His tag)Crystallography grade ATG10 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Autophagy Related 10 (ATG10) (AA 1-215) protein (His tag)Crystallography grade ATG10 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Autophagy Related 10 (ATG10) (Transcript Variant 3) protein (His tag)ATG10 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- ATG10 (Autophagy Related 10 (ATG10))
- Alternative Name
- Ubiquitin-Like-Conjugating Enzyme ATG10/ATG10 (ATG10 Products)
- Background
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Recombinant Human Ubiquitin-Like-Conjugating Enzyme ATG10/ATG10 produced by E. coli expression system. The target protein is expressed with sequence (Met1-Thr190) of Human ATG10 fused with a 6His tag at the C-terminus.
Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28 kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7. - Molecular Weight
- 24.5 kDa
- UniProt
- Q9H0Y0
- Pathways
- ER-Nucleus Signaling, Autophagy
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