BDH2 Protein (AA 1-245) (His tag)

Catalog No. ABIN1096095

Product Details

Target: BDH2 (3-hydroxybutyrate Dehydrogenase, Type 2 (BDH2))

Protein Type: Recombinant

Protein Characteristics: AA 1-245

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BDH2 protein is labelled with His tag.

Purpose: Recombinant Human 3-Hydroxybutyrate Dehydrogenase Type 2/BDH2/DHRS6 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAS EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL

Characteristics: Recombinant Human 3-Hydroxybutyrate Dehydrogenase Type 2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu245) of Human BDH2 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: BDH2 (3-hydroxybutyrate Dehydrogenase, Type 2 (BDH2))

Alternative Name: BDH2 (BDH2 Products)

Synonyms: DHRS6 Protein, BDH2 Protein, bdh2 Protein, EFA6R Protein, PRO20933 Protein, SDR15C1 Protein, UCPA-OR Protein, UNQ6308 Protein, 1810026B04Rik Protein, Dhrs6 Protein, zgc:110323 Protein, 3-hydroxybutyrate dehydrogenase, type 2 Protein, 3-hydroxybutyrate dehydrogenase 2 Protein, 3-hydroxybutyrate dehydrogenase 2 L homeolog Protein, BDH2 Protein, bdh2 Protein, Bdh2 Protein, bdh2.L Protein

Sub Type: Fusionprotein

Background: 3-Hydroxybutyrate Dehydrogenase Type 2 belongs to the short-chain dehydrogenases/reductases (SDR) family. 3-Hydroxybutyrate Dehydrogenase Type 2 may play an important role in the peripheral utilization of 3-hydroxybutyrate. The cytoplasmic localization with its high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters of 3-Hydroxybutyrate Dehydrogenase Type 2 make it suitable to conbert high levels of circulating 3-hydroxybutyrate into acetoacetate.
Alternative Names: 3-Hydroxybutyrate Dehydrogenase Type 2, Dehydrogenase/Reductase SDR Family Member 6, Oxidoreductase UCPA, R-Beta-Hydroxybutyrate Dehydrogenase, BDH2, DHRS6

Molecular Weight: 28.9 kDa

UniProt: Q9BUT1

Pathways: Transition Metal Ion Homeostasis, Monocarboxylic Acid Catabolic Process