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NGFB Protein (AA 122-241)

NGFB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Func Active
Catalog No. ABIN1096128
  • Target See all NGFB Proteins
    NGFB (Nerve Growth Factor beta (NGFB))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 122-241
    Origin
    • 7
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 8
    • 4
    • 3
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Application
    Functional Studies (Func)
    Purpose
    Recombinant Human β-Nerve Growth Factor/β-NGF (Ser122-Ala241, E. coli)
    Sequence
    SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
    Characteristics
    Recombinant Human NGF Beta (beta-Nerve Growth Factor/beta-NGF) produced in E. coli is a non-glycosylated non-covalent homodimer of two 118 amino acid polypeptides each with a molecular mass of 13.4 kDa.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
    Top Product
    Discover our top product NGFB Protein
  • Comment

    Biological activity: ED50 is less than 1.0 ng/ml as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.Specific Activity is greater than 1 x 106 IU/mg.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.0.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Storage
    4 °C/-20 °C/-80 °C
    Storage Comment
    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.
    Expiry Date
    3 months
  • Target
    NGFB (Nerve Growth Factor beta (NGFB))
    Alternative Name
    beta-Nerve Growth Factor (NGFB Products)
    Synonyms
    NGFB Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, hsan5 Protein, beta-ngf Protein, Ngfb Protein, Beta-NGF Protein, HSAN5 Protein, beta-NGF Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, beta-Nerve growth factor Protein, nerve growth factor (beta polypeptide) Protein, NGF Protein, ngfb Protein, FPV072 Protein, FPV076 Protein, ngf Protein, Ngf Protein
    Background
    Recombinant Human NGF Beta: beta-Nerve Growth Factor/beta-NGF was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits alpha, beta, and gamma, it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Beta-NGF is a neurotrophic factor that signals through its receptor beta-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that beta-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Recombinant Human NGF Beta shares 90% sequence similarity with mouse protein and shows cross-species reactivity.ReferencesForsell P, et al. The use of TrkA-PathHunter assay in high-throughput screening to identify compounds that affect nerve growth factor signaling.PMID: 23458757http://www.ncbi.nlm.nih.gov/pubmed/23458757
    Alternative Names: Recombinant Human NGF Beta, Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB
    Molecular Weight
    13.4 kDa
    UniProt
    P01138
    Pathways
    NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size
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