CA1 Protein (AA 2-261) (His tag)

Catalog No. ABIN1096194

Product Details

Target: CA1 (Carbonic Anhydrase I (CA1))

Protein Type: Recombinant

Protein Characteristics: AA 2-261

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CA1 protein is labelled with His tag.

Purpose: Recombinant Human Carbonic Anhydrase 1/CA1 (C-6His)

Sequence: ASPDWGYDDK NGPEQWSKLY PIANGNNQSP VDIKTSETKH DTSLKPISVS YNPATAKEII NVGHSFHVNF EDNDNRSVLK GGPFSDSYRL FQFHFHWGST NEHGSEHTVD GVKYSAELHV AHWNSAKYSS LAEAASKADG LAVIGVLMKV GEANPKLQKV LDALQAIKTK GKRAPFTNFD PSTLLPSSLD FWTYPGSLTH PPLYESVTWI ICKESISVSS EQLAQFRSLL SNVEGDNAVP MQHNNRPTQP LKGRTVRASF LEHHHHHH

Characteristics: Recombinant Human Carbonic Anhydrase 1/CA1 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Phe261) of Human CA1.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CA1 (Carbonic Anhydrase I (CA1))

Alternative Name: Carbonic Anhydrase 1/CA1 (CA1 Products)

Synonyms: CA-I Protein, CAB Protein, Car1 Protein, AW555628 Protein, Ca1 Protein, Car-1 Protein, BG:DS00941.1 Protein, CAH Protein, CG7820 Protein, Dmel\\CG7820 Protein, Gh7 Protein, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 Protein, ATBCA1 Protein, ATSABP3 Protein, BETA CARBONIC ANHYDRASE 1 Protein, F4P13.5 Protein, F4P13_5 Protein, SABP3 Protein, SALICYLIC ACID-BINDING PROTEIN 3 Protein, carbonic anhydrase 1 Protein, CA1 Protein, LOC100135826 Protein, GB15888 Protein, carbonic anhydrase 1 Protein, Carbonic anhydrase 1 Protein, carbonic anhydrase I Protein, carbonic anhydrase Protein, CA1 Protein, Car1 Protein, CAH1 Protein, LOC100135826 Protein, LOC408827 Protein, Tsp_05114 Protein, LOC100153915 Protein, LOC100050192 Protein, Ca1 Protein

Background: Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.
Alternative Names: Carbonic Anhydrase 1, Carbonate Dehydratase I, Carbonic Anhydrase B, CAB, Carbonic Anhydrase I, CA-I, CA1

Molecular Weight: 29.93 kDa

UniProt: P00915