CLIC4 Protein (AA 1-253) (His tag)

Catalog No. ABIN1096389

Product Details

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Protein Type: Recombinant

Protein Characteristics: AA 1-253

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CLIC4 protein is labelled with His tag.

Purpose: Recombinant Human Chloride Intracellular Channel Protein 4/CLIC4 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV VFSVTTVDLK RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC PPKYLKLSPK HPESNTAGMD IFAKFSAYIK NSRPEANEAL ERGLLKTLQK LDEYLNSPLP DEIDENSMED IKFSTRKFLD GNEMTLADCN LLPKLHIVKV VAKKYRNFDI PKEMTGIWRY LTNAYSRDEF TNTCPSDKEV EIAYSDVAKR LTK

Characteristics: Recombinant Human Chloride Intracellular Channel Protein 4/CLIC4 produced by E. coli expression system. The target protein is expressed with sequence (Met1-Lys253) of Human CLIC4 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Alternative Name: Chloride Intracellular Channel Protein 4/CLIC4 (CLIC4 Products)

Synonyms: CLIC4L Protein, H1 Protein, MTCLIC Protein, huH1 Protein, p64H1 Protein, cb142 Protein, wu:fb33c05 Protein, wu:fc30h10 Protein, clic4 Protein, MGC89006 Protein, CLIC4 Protein, DKFZp459A0325 Protein, D0Jmb3 Protein, TU-74 Protein, mc3s5 Protein, mtCLIC Protein, xclic4 Protein, chloride intracellular channel 4 Protein, chloride intracellular channel 4 (mitochondrial) Protein, chloride intracellular channel 4 S homeolog Protein, CLIC4 Protein, Clic4 Protein, clic4 Protein, clic4.S Protein

Sub Type: Fusionprotein

Background: Chloride Intracellular Channel Protein 4 (CLIC4) is a 253 amino acid single-pass membrane protein that localizes to both the nucleus and the cytoplasm and contains one GST C-terminal domain. CLIC4 is expressed in various tissues and exhibits an intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). CLIC4 acts as a monomer which is able to form selective ion channels in target proteins, thus facilitating the transport of chloride and other ions. CLIC4 is believed to have a role in apoptosis and is able to translocate to the nucleus under stress conditions. CLIC4 has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis.
Alternative Names: Chloride Intracellular Channel Protein 4, Intracellular Chloride Ion Channel Protein p64H1, CLIC4

Molecular Weight: 30.9 kDa

UniProt: Q9Y696