CSTA Protein (AA 2-98) (His tag)
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- Target See all CSTA Proteins
- CSTA (Cystatin A (Stefin A) (CSTA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-98
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CSTA protein is labelled with His tag.
- Purpose
- Recombinant Human Cystatin A (N-6His)
- Sequence
- MHHHHHHIPG GLSEAKPATP EIQEIVDKVK PQLEEKTNET YGKLEAVQYK TQVVAGTNYY IKVRAGDNKY MHLKVFKSLP GQNEDLVLTG YQVDKNKDDE LTGF
- Characteristics
- Recombinant Human Cystatin A is produced with our E. coli expression system. The target protein is expressed with sequence (Ile2-Phe98) of Human Cystatin A.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CSTA Protein
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Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (GST tag)
CSTA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin A (Stefin A) (CSTA) (AA 1-97) protein (Strep Tag)Crystallography grade CSTA Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Cystatin A (Stefin A) (CSTA) (AA 2-97) protein (His tag)Crystallography grade CSTA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- CSTA (Cystatin A (Stefin A) (CSTA))
- Alternative Name
- cystatin-a (CSTA Products)
- Synonyms
- AREI Protein, STF1 Protein, STFA Protein, CST Protein, CSTA Protein, ATCYSA Protein, FL3-27 Protein, T20B5.8 Protein, T20B5_8 Protein, cystatin A Protein, cystatin A Protein, cystatin A1 Protein, cystatin-A Protein, CSTA Protein, Csta1 Protein, Csta Protein, CYSA Protein, LOC100725920 Protein
- Background
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Human Cystatin A (CSTA) is a member of family 1 of the cystatin superfamily, which is characterized by lacking of disulphide bonds an carbohydrates. Cystatin A is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as Cathepsins B, H and L. Cystatin A is also implicated in a number of disease states. Due to altered proteolytic state in cancer progression, Cystatin A may play a role in the proteolytic pathways.
Alternative Names: Cystatin-A, Cystatin-AS, Stefin-A, CSTA, STF1, STFA - Molecular Weight
- 11.8 kDa
- UniProt
- P01040
- Pathways
- Response to Water Deprivation
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