EPT1 Protein (AA 1-50) (GST tag)
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- Target See all EPT1 Proteins
- EPT1 (ethanolaminephosphotransferase 1 (CDP-Ethanolamine-Specific) (EPT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-50
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EPT1 protein is labelled with GST tag.
- Purpose
- Recombinant Human Ethanolaminephosphotransferase 1/EPT1 (N-GST)
- Sequence
- MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFH MAGYEYVSPE QLAGFDKYKY SAVDTNPLSL YVMHPFWNTI VKVFPTWLAP
- Characteristics
- Recombinant Human Ethanolaminephosphotransferase 1/EPT1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Pro50) of Human EPT1 fused with a GST tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product EPT1 Protein
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ethanolaminephosphotransferase 1 (CDP-Ethanolamine-Specific) (EPT1) (AA 1-26) protein (His tag)
EPT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ethanolaminephosphotransferase 1 (CDP-Ethanolamine-Specific) (EPT1) (AA 1-50) protein (GST tag)EPT1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
ethanolaminephosphotransferase 1 (CDP-Ethanolamine-Specific) (EPT1) (AA 2-397) protein (rho-1D4 tag)Crystallography grade EPT1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
ethanolaminephosphotransferase 1 (CDP-Ethanolamine-Specific) (EPT1) (AA 2-398) protein (rho-1D4 tag)Crystallography grade EPT1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- EPT1 (ethanolaminephosphotransferase 1 (CDP-Ethanolamine-Specific) (EPT1))
- Alternative Name
- hEPT1 (EPT1 Products)
- Synonyms
- SELI Protein, SEPI Protein, 4933402G07Rik Protein, AI448296 Protein, AI452230 Protein, C79563 Protein, D5Wsu178e Protein, mKIAA1724 Protein, hEPT1 Protein, RGD1560938 Protein, EPT1 Protein, selenoprotein I Protein, SELENOI Protein, Selenoi Protein
- Sub Type
- Fusionprotein
- Background
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Ethanolaminephosphotransferase 1 (EPT1) is an enzyme that belongs to the CDP-Alcohol Phosphatidyltransferase Class-I Family. EPT1 is a Selenoprotein, which contains a Selenocysteine (Sec) residue at its active site. The Selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. EPT1 catalyzes Phosphatidylethanolamine biosynthesis from CDP-Ethanolamine. It plays a central role in the formation and maintenance of vesicular membranes. EPT1 is involved in the formation of Phosphatidylethanolamine via the 'Kennedy' pathway.
Alternative Names: Ethanolaminephosphotransferase 1, hEPT1, Selenoprotein I, SelI, EPT1, KIAA1724, SELI - Molecular Weight
- 32.3 kDa
- UniProt
- Q9C0D9
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