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ASF1A Protein (AA 1-204) (His tag,T7 tag)

ASF1A Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Catalog No. ABIN1096822
  • Target See all ASF1A Proteins
    ASF1A (ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-204
    Origin
    • 5
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 5
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This ASF1A protein is labelled with His tag,T7 tag.
    Purpose
    Recombinant Human Histone Chaperone ASF1A/ASF1A (C-6His, N-T7 tag)
    Sequence
    MASMTGGQQM GRGSMAKVQV NNVVVLDNPS PFYNPFQFEI TFECIEDLSE DLEWKIIYVG SAESEEYDQV LDSVLVGPVP AGRHMFVFQA DAPNPGLIPD ADAVGVTVVL ITCTYRGQEF IRVGYYVNNE YTETELRENP PVKPDFSKLQ RNILASNPRV TRFHINWEDN TEKLEDAESS NPNLQSLLST DALPSASKGW STSENSLNVM LESHMDCMLE HHHHHH
    Characteristics
    Recombinant Human Histone Chaperone ASF1A/ASF1A is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Met204) of Human ASF1A fused with a 6His tag at the C-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
    Top Product
    Discover our top product ASF1A Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 150 mM NaCl, pH 8.0.
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Storage
    4 °C/-20 °C/-80 °C
    Storage Comment
    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.
    Expiry Date
    3 months
  • Target
    ASF1A (ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A))
    Alternative Name
    Histone Chaperone ASF1A/ASF1A (ASF1A Products)
    Synonyms
    CGI-98 Protein, CIA Protein, HSPC146 Protein, 2310079C17Rik Protein, C79052 Protein, ASF1 Protein, ASF1A Protein, XAsf1 Protein, asf1 Protein, asf1-a Protein, asf1a Protein, asf1a-a Protein, asf1aa Protein, asf1ab Protein, anti-silencing function 1A histone chaperone Protein, anti-silencing function 1A histone chaperone L homeolog Protein, ASF1A Protein, Asf1a Protein, asf1a.L Protein
    Sub Type
    Fusionprotein
    Background
    Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
    Alternative Names: Histone Chaperone ASF1A, Anti-Silencing Function Protein 1 Homolog A, hAsf1, hAsf1a, CCG1-Interacting Factor A, CIA, hCIA, ASF1A
    Molecular Weight
    25.4 kDa
    UniProt
    Q9Y294
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