F11R Protein (AA 28-238) (His tag)

Catalog No. ABIN1096993

Product Details

Target: F11R (F11 Receptor (F11R))

Protein Type: Recombinant

Protein Characteristics: AA 28-238

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This F11R protein is labelled with His tag.

Purpose: Recombinant Human Junctional Adhesion Molecule A/JAM-A/CD321 (C-6His)

Sequence: SVTVHSSEPE VRIPENNPVK LSCAYSGFSS PRVEWKFDQG DTTRLVCYNN KITASYEDRV TFLPTGITFK SVTREDTGTY TCMVSEEGGN SYGEVKVKLI VLVPPSKPTV NIPSSATIGN RAVLTCSEQD GSPPSEYTWF KDGIVMPTNP KSTRAFSNSS YVLNPTTGEL VFDPLSASDT GEYSCEARNG YGTPMTSNAV RMEAVERNVG VDHHHHHH

Characteristics: Recombinant Human Junctional Adhesion Molecule A/JAM-A produced by transfected human cells is a secreted protein with sequence (Ser28-Val238) of Human JAM-A fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 50 mM NaCl, 100 mM Glycine, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: F11R (F11 Receptor (F11R))

Alternative Name: jam-a (F11R Products)

Synonyms: CD321 Protein, JAM Protein, JAM1 Protein, JAMA Protein, JCAM Protein, KAT Protein, PAM-1 Protein, 9130004G24 Protein, AA638916 Protein, ESTM33 Protein, JAM-1 Protein, JAM-A Protein, Jcam Protein, Jcam1 Protein, Ly106 Protein, Jam1 Protein, F11 receptor Protein, F11R Protein, F11r Protein

Sub Type: Fusionprotein

Background: Junctional Adhesion Molecule A (JAM-A) is a single-pass type I membrane protein that belongs to the immunoglobulin superfamily. JAM-A contains 2 Ig-like V-type (immunoglobulin-like) domains and Interacts with the ninth PDZ domain. JAM-A is localized to the tight junctions of both epithelial and endothelial cells. JAM-A seems to be involved in epithelial tight junction formation. JAM-A appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM-A, thereby preventing tight junction assembly. JAM-A plays a role in regulating monocyte transmigration involved in regulating integrity of the epithelial barrier. In the case of orthoreovirus infection, JAM-A serves as receptor for the virus.
Alternative Names: Junctional Adhesion Molecule A, JAM-A, Junctional Adhesion Molecule 1, JAM-1, Platelet F11 Receptor, Platelet Adhesion Molecule 1, PAM-1, CD321, F11R, JAM1, JCAM

Molecular Weight: 23.89 kDa

UniProt: Q9Y624

Pathways: Cell-Cell Junction Organization