KPNA2 Protein (AA 1-529) (His tag)

Catalog No. ABIN1097041

Product Details

Target: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Protein Type: Recombinant

Protein Characteristics: AA 1-529

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This KPNA2 protein is labelled with His tag.

Purpose: Recombinant Human Karyopherin Subunit α-2 /KPNA2/IPOA1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MSTNENANTP AARLHRFKNK GKDSTEMRRR RIEVNVELRK AKKDDQMLKR RNVSSFPDDA TSPLQENRNN QGTVNWSVDD IVKGINSSNV ENQLQATQAA RKLLSREKQP PIDNIIRAGL IPKFVSFLGR TDCSPIQFES AWALTNIASG TSEQTKVVVD GGAIPAFISL LASPHAHISE QAVWALGNIA GDGSVFRDLV IKYGAVDPLL ALLAVPDMSS LACGYLRNLT WTLSNLCRNK NPAPPIDAVE QILPTLVRLL HHDDPEVLAD TCWAISYLTD GPNERIGMVV KTGVVPQLVK LLGASELPIV TPALRAIGNI VTGTDEQTQV VIDAGALAVF PSLLTNPKTN IQKEATWTMS NITAGRQDQI QQVVNHGLVP FLVSVLSKAD FKTQKEAVWA VTNYTSGGTV EQIVYLVHCG IIEPLMNLLT AKDTKIILVI LDAISNIFQA AENLGETEKL SIMIEECGGL DKIEALQNHE NESVYKASLS LIEKYFSVEE EEDQNVVPET TSEGYTFQVQ DGAPGTFNF

Characteristics:

Recombinant Human Karyopherin Subunit α-2 /KPNA2/IPOA1 (N-6His)

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,1 mM DTT,20 % Glycerol, pH 8.0 .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Alternative Name: Karyopherin Subunit alpha-2 /KPNA2 (KPNA2 Products)

Synonyms: IPOA1 Protein, QIP2 Protein, RCH1 Protein, SRP1alpha Protein, ima2 Protein, importin Protein, 2410044B12Rik Protein, PTAC58 Protein, Rch1 Protein, ipoa1 Protein, kpna2 Protein, qip2 Protein, rch1 Protein, srp1alpha Protein, hm:zeh0389 Protein, hm:zeh0389r Protein, zgc:113902 Protein, zgc:86945 Protein, karyopherin subunit alpha 2 Protein, importin subunit alpha-2 Protein, Importin subunit alpha-2 Protein, karyopherin (importin) alpha 2 Protein, karyopherin alpha-2 subunit like L homeolog Protein, karyopherin alpha 2 (RAG cohort 1, importin alpha 1) Protein, KPNA2 Protein, EDI_246290 Protein, EDI_335040 Protein, ima2 Protein, Kpna2 Protein, kpna2.L Protein, kpna2 Protein

Background: Recombinant Human Karyopherin Subunit α-2/KPNA2 is produced by our E. coli expression system. The target protein is expressed with sequence (Ser2-Phe529) of Human KPNA2 fused with a 6His tag at the N-terminus.
Karyopherin Subunit α-2 (KPNA2) belongs to the importin α family. KPNA2 is widely expressed in many tissues and contains an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. KPNA2 interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. KPNA2 functions in nuclear protein importantly as an adapter protein for nuclear receptor KPNB1.

Molecular Weight: 60.04 kDa

UniProt: P52292

Pathways: M Phase, Protein targeting to Nucleus