LGMN Protein (AA 18-433) (His tag)

Catalog No. ABIN1097074

Product Details

Target: LGMN (Legumain (LGMN))

Protein Type: Recombinant

Protein Characteristics: AA 18-433

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This LGMN protein is labelled with His tag.

Purpose: Recombinant Human Legumain/Asparaginyl Endopeptidase (C-6His)

Sequence: IPIDDPEDGG KHWVVIVAGS NGWYNYRHQA DACHAYQIIH RNGIPDEQIV VMMYDDIAYS EDNPTPGIVI NRPNGTDVYQ GVPKDYTGED VTPQNFLAVL RGDAEAVKGI GSGKVLKSGP QDHVFIYFTD HGSTGILVFP NEDLHVKDLN ETIHYMYKHK MYRKMVFYIE ACESGSMMNH LPDNINVYAT TAANPRESSY ACYYDEKRST YLGDWYSVNW MEDSDVEDLT KETLHKQYHL VKSHTNTSHV MQYGNKTIST MKVMQFQGMK RKASSPVPLP PVTHLDLTPS PDVPLTIMKR KLMNTNDLEE SRQLTEEIQR HLDARHLIEK SVRKIVSLLA ASEAEVEQLL SERAPLTGHS CYPEALLHFR THCFNWHSPT YEYALRHLYV LVNLCEKPYP LHRIKLSMDH VCLGHYVDHH HHHH

Characteristics: Recombinant Human Legumain produced by transfected human cells is a secreted protein with sequence (Ile18-Tyr433) of Human Legumain fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: LGMN (Legumain (LGMN))

Alternative Name: legumain (LGMN Products)

Synonyms: AEP Protein, LGMN1 Protein, PRSC1 Protein, AI746452 Protein, AU022324 Protein, Prsc1 Protein, MGC64351 Protein, cb173 Protein, cb460 Protein, sb:cb173 Protein, zgc:76953 Protein, wu:fa98g05 Protein, wu:fj92g08 Protein, lgmn Protein, MGC88980 Protein, DELTA VACUOLAR PROCESSING ENZYME Protein, DELTAVPE Protein, delta vacuolar processing enzyme Protein, legumain Protein, legumain L homeolog Protein, delta vacuolar processing enzyme Protein, Legumain Protein, LGMN Protein, Lgmn Protein, lgmn.L Protein, lgmn Protein, DELTA-VPE Protein, MBOO_RS07785 Protein, Odosp_3582 Protein, LOC569153 Protein

Sub Type: Fusionprotein

Background: Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.
Alternative Names: Legumain, Asparaginyl Endopeptidase, Protease Cysteine 1, LGMN, PRSC1

Molecular Weight: 48.72 kDa

UniProt: Q99538

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades