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NADK Protein (AA 64-446) (His tag)

NADK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Catalog No. ABIN1097191
  • Target See all NADK Proteins
    NADK (NAD Kinase (NADK))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 64-446
    Origin
    • 10
    • 1
    • 1
    • 1
    Human
    Source
    • 8
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This NADK protein is labelled with His tag.
    Purpose
    Recombinant Human NAD Kinase/NADK (N-6His)
    Sequence
    MGSSHHHHHH SSGLVPRGSH MSLHGPCPVT TFGPKACVLQ NPQTIMHIQD PASQRLTWNK SPKSVLVIKK MRDASLLQPF KELCTHLMEE NMIVYVEKKV LEDPAIASDE SFGAVKKKFC TFREDYDDIS NQIDFIICLG GDGTLLYASS LFQGSVPPVM AFHLGSLGFL TPFSFENFQS QVTQVIEGNA AVVLRSRLKV RVVKELRGKK TAVHNGLGEK GSQAAGLDMD VGKQAMQYQV LNEVVIDRGP SSYLSNVDVY LDGHLITTVQ GDGVIVSTPT GSTAYAAAAG ASMIHPNVPA IMITPICPHS LSFRPIVVPA GVELKIMLSP EARNTAWVSF DGRKRQEIRH GDSISITTSC YPLPSICVRD PVSDWFESLA QCLHWNVRKK QAHFEEEEEE EEEG
    Characteristics
    Recombinant Human NAD Kinase/NADK is produced by our E. coli expression system. The target protein is expressed with sequence (Ser64-Gly446) of Human NADK fused with a 6His tag at the N-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
    Top Product
    Discover our top product NADK Protein
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, 150 mM NaCl, 1 mM DTT, pH 7.5.
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Storage
    -80 °C
    Storage Comment
    Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.
    Expiry Date
    6 months
  • Target
    NADK (NAD Kinase (NADK))
    Alternative Name
    NAD Kinase/NADK (NADK Products)
    Synonyms
    4432404C02Rik Protein, BC004012 Protein, dJ283E3.1 Protein, NAD kinase Protein, Nadk Protein, NADK Protein
    Sub Type
    Fusionprotein
    Background
    NAD Kinase (NADK) is an enzyme that belongs to the NAD Kinase family. It is a widely expressed enzyme, but it is not detected in skeletal muscle. NADK converts Nicotinamide Adenine Dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined.
    Alternative Names: NAD Kinase, Poly(P)/ATP NAD Kinase, NADK
    Molecular Weight
    44.4 kDa
    UniProt
    O95544
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