PVRL1 Protein (AA 31-334) (His tag)

Catalog No. ABIN1097203

Product Details

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Protein Type: Recombinant

Protein Characteristics: AA 31-334

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This PVRL1 protein is labelled with His tag.

Purpose: Recombinant Human Poliovirus Receptor-Related Protein 1/PVRL1 (C-6His)

Sequence: QVVQVNDSMY GFIGTDVVLH CSFANPLPSV KITQVTWQKS TNGSKQNVAI YNPSMGVSVL APYRERVEFL RPSFTDGTIR LSRLELEDEG VYICEFATFP TGNRESQLNL TVMAKPTNWI EGTQAVLRAK KGQDDKVLVA TCTSANGKPP SVVSWETRLK GEAEYQEIRN PNGTVTVISR YRLVPSREAH QQSLACIVNY HMDRFKESLT LNVQYEPEVT IEGFDGNWYL QRMDVKLTCK ADANPPATEY HWTTLNGSLP KGVEAQNRTL FFKGPINYSL AGTYICEATN PIGTRSGQVE VNITVDHHHH HH

Characteristics: Recombinant Human Poliovirus Receptor-Related Protein 1/PVRL1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Gln31-Thr334) of Human PVRL1 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Alternative Name: nectin-1 (PVRL1 Products)

Synonyms: CD111 Protein, CLPED1 Protein, ED4 Protein, HIgR Protein, HVEC Protein, OFC7 Protein, PRR Protein, PRR1 Protein, PVRR Protein, PVRR1 Protein, SK-12 Protein, nectin-1 Protein, prr1 Protein, PVRL1 Protein, pvrl1 Protein, AI835281 Protein, AW549174 Protein, Cd111 Protein, HveC Protein, cd111 Protein, cd112 Protein, clped1 Protein, ed4 Protein, higr Protein, hveb Protein, hvec Protein, nectin-2 Protein, ofc7 Protein, prr Protein, prr2 Protein, pvrl2 Protein, pvrl2.L Protein, pvrr Protein, pvrr1 Protein, pvrr2 Protein, sk-12 Protein, nectin cell adhesion molecule 1 Protein, nectin-1 Protein, Poliovirus receptor-related protein 1 Protein, poliovirus receptor-related 1 (herpesvirus entry mediator C) Protein, nectin cell adhesion molecule 2 L homeolog Protein, NECTIN1 Protein, LOC100145553 Protein, pvrl1 Protein, nectin1 Protein, Nectin1 Protein, PVRL1 Protein, nectin2.L Protein

Sub Type: Fusionprotein

Background: Nectin-1 is a type I transmembrane glycoprotein belonging to the Ig superfamily. Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Nectin ECDs contain three Ig like domains: an N terminal V type that mediates ligand binding, and two C2 type. Nectin-1 binds viral Glycoprotein D to mediate Herpesvirus (but not Poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts. In forming adherens junctions and synapses, Nectin-1 and Nectin-3 initiate cell-cell interactions, recruiting alphavbeta3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins. These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 and Nectin-3 have been found to localize assymetrically along the chemical synapse, with Nectin-1 primarily on the axonal side and Nectin-3 on the dendritic side. Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia. Nectin-1 downregulation in epithelial cancers is mediated in part by ectodomain shedding, but it may contribute to invasiveness.
Alternative Names: Poliovirus Receptor-Related Protein 1, Herpes Virus Entry Mediator C, Herpesvirus Entry Mediator C, HveC, Herpesvirus Ig-Like Receptor, HIgR, Nectin-1, CD111, PVRL1, HVEC, PRR1

Molecular Weight: 34.99 kDa

UniProt: Q15223

Pathways: Cell-Cell Junction Organization