PAFAH1B2 Protein (AA 2-229) (His tag)
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- Target See all PAFAH1B2 Proteins
- PAFAH1B2 (Platelet-Activating Factor Acetylhydrolase 1b, Catalytic Subunit 2 (30kDa) (PAFAH1B2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-229
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PAFAH1B2 protein is labelled with His tag.
- Purpose
- Recombinant Human PAF-AH subunit β/PAFAHB (C-6His)
- Sequence
- SQGDSNPAAI PHAAEDIQGD DRWMSQHNRF VLDCKDKEPD VLFVGDSMVQ LMQQYEIWRE LFSPLHALNF GIGGDTTRHV LWRLKNGELE NIKPKVIVVW VGTNNHENTA EEVAGGIEAI VQLINTRQPQ AKIIVLGLLP RGEKPNPLRQ KNAKVNQLLK VSLPKLANVQ LLDTDGGFVH SDGAISCHDM FDFLHLTGGG YAKICKPLHE LIMQLLEETP EEKQTTIAVE HHHHHH
- Characteristics
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Recombinant Human PAF-AH subunit β/PAFAHB (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PAFAH1B2 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PAFAH1B2 (Platelet-Activating Factor Acetylhydrolase 1b, Catalytic Subunit 2 (30kDa) (PAFAH1B2))
- Alternative Name
- Platelet-Activating Factor Acetylhydrolase IB Subunit beta/pafahb (PAFAH1B2 Products)
- Background
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Recombinant Human Platelet-Activating Factor Acetylhydrolase IB Subunit β/PAFAHB is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala229) of Human PAFAHB fused with a His tag at the C-terminus.
Platelet-Activating Factor Acetylhydrolase IB Subunit β (PAFAHB) is a cytoplasmic hydrolase. PAFAHB is a member of the GDSL lipolytic enzyme family. It also belongs to Platelet-activating factor acetylhydrolase IB β/γ subunits subfamily. Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (α), 30 kDa (β) and 29 kDa (γ), PAFAHB is a catalytic subunit. PAFAHB inactivates PAF by removing the acetyl group at the sn-2 position. - Molecular Weight
- 26.6 kDa
- UniProt
- P68402
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