PYM Protein (AA 1-204) (His tag)
-
- Target See all PYM (WIBG) Proteins
- PYM (WIBG) (Partner of Y14 and mago (WIBG))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-204
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PYM protein is labelled with His tag.
- Purpose
- Recombinant Human Partner of Y14 and Mago/WIBG /PYM (C-6His)
- Sequence
- MEAAGSPAAT ETGKYIASTQ RPDGTWRKQR RVKEGYVPQE EVPVYENKYV KFFKSKPELP PGLSPEATAP VTPSRPEGGE PGLSKTAKRN LKRKEKRRQQ QEKGEAEALS RTLDKVSLEE TAQLPSAPQG SRAAPTAASD QPDSAATTEK AKKIKNLKKK LRQVEELQQR IQAGEVSQPS KEQLEKLARR RALEEELEDL ELGLLEHHHH HH
- Characteristics
- Recombinant Human Partner of Y14 and Mago/WIBG is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu204) of Human WIBG fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product WIBG Protein
-
Partner of Y14 and mago (WIBG) (AA 1-204) protein (GST tag)
WIBG Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Partner of Y14 and mago (WIBG) (AA 1-203) protein (Strep Tag)Crystallography grade WIBG Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Partner of Y14 and mago (WIBG) (Transcript Variant 2) protein (His tag)WIBG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Partner of Y14 and mago (WIBG) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)WIBG Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Partner of Y14 and mago (WIBG) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)WIBG Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 10 % Glycerol, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
-
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
-
- Target
- PYM (WIBG) (Partner of Y14 and mago (WIBG))
- Alternative Name
- WIBG (WIBG Products)
- Synonyms
- BEST:HL05757 Protein, CG10330 Protein, CG30176 Protein, Dmel\\CG30176 Protein, PYM Protein, Pym Protein, UD3 Protein, A030010B05Rik Protein, zgc:65891 Protein, zgc:77281 Protein, Partner of Y14 and Mago Protein, Wibg protein Protein, PYM homolog 1, exon junction complex associated factor L homeolog Protein, PYM homolog 1, exon junction complex associated factor Protein, Pym Protein, wibg Protein, pym1.L Protein, PYM1 Protein, Pym1 Protein, pym1 Protein
- Sub Type
- Fusionprotein
- Background
-
Partner of Y14 and Mago (WIBG) is a key regulator of the Exon Junction Complex (EJC). EJC is a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs, is a positional landmarker for the intron exon structure of genes, and directs post-transcriptional processes in the cytoplasm, for instance mRNA export, nonsense-mediated mRNA decay or translation. WIBG is a cytoplasmic RNA-binding protein, it can be excluded from nucleus by Crm1. WIBG as a cooperateing partner of Mago-14, relates with Mago-14 by its N-terminal domain.
Alternative Names: Partner of Y14 and Mago, Protein Wibg Homolog, WIBG, PYM - Molecular Weight
- 23.7 kDa
- UniProt
- Q9BRP8
-
