PLA2G7 Protein (AA 22-441) (His tag)
-
- Target See all PLA2G7 (Lp-PLA2) Proteins
- PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-441
-
Origin
- Human
-
Source
- Human Cells
- Purification tag / Conjugate
- This PLA2G7 protein is labelled with His tag.
- Purpose
- Recombinant Human PAF-AH/PLA2G7 (C-6His)
- Sequence
- FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNAAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN VDHHHHHH
- Characteristics
- Recombinant Human Platelet-Activating Factor Acetylhydrolase/PAFAH is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Phe22-Asn441) of Human PAFAH fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product Lp-PLA2 Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM HAc-NaCl, 150 mM NaCl, 10 % Glycerol, pH 4.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
-
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
-
- Target
- PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))
- Alternative Name
- lp-pla2 (Lp-PLA2 Products)
- Synonyms
- LDL-PLA2 Protein, LP-PLA2 Protein, PAFAD Protein, PAFAH Protein, Lp-PLA2 Protein, PAF-AH Protein, R75400 Protein, zgc:77563 Protein, phospholipase A2 group VII Protein, phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) Protein, PLA2G7 Protein, Pla2g7 Protein, pla2g7 Protein
- Sub Type
- Fusionprotein
- Background
-
Platelet-Activating Factor Acetylhydrolase (PAFAH) is a secreted enzyme which belongs to the AB hydrolase superfamily and Lipase family and catalyzes the degradation of platelet-activating factor to biologically inactive products. PAFAH is produced by inflammatory cells and hydrolyzes oxidised phospholipids in LDL. PAFAH has been implicated in the development of atherosclerosis and has also been identified as a marker for cardiac disease. PAFAH might have a major physiologic effect in the presence of inflammatory bodily responses. PAFAH alters the action of PAF by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. PAFAH has specificity for substrates with a short residue at the sn-2 position.
Alternative Names: Platelet-Activating Factor Acetylhydrolase, PAF Acetylhydrolase, 1-Alkyl-2-Acetylglycerophosphocholine Esterase, 2-Acetyl-1-Alkylglycerophosphocholine Esterase, Group-VIIA Phospholipase A2, gVIIA-PLA2, LDL-Associated Phospholipase A2, LDL-PLA(2), PAF 2-Ac - Molecular Weight
- 48.8 kDa
- UniProt
- Q13093
- Pathways
- Peptide Hormone Metabolism
-