PMM1 Protein (AA 1-262) (His tag)
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- Target See all PMM1 Proteins
- PMM1 (Phosphomannomutase 1 (PMM1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-262
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PMM1 protein is labelled with His tag.
- Purpose
- Recombinant Human Phosphomannomutase 1/PMM1 (C-6His)
- Sequence
- MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC REIFFPETAH EAVEHHHHHH
- Characteristics
- Recombinant Human Phosphomannomutase 1/PMM1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala262) of Human PMM1 fused with a His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PMM1 Protein
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Phosphomannomutase 1 (PMM1) (AA 1-262) protein (His tag)
PMM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Phosphomannomutase 1 (PMM1) (AA 1-262) protein (GST tag)PMM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Phosphomannomutase 1 (PMM1) (AA 2-262) protein (His tag)Crystallography grade PMM1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Phosphomannomutase 1 (PMM1) (AA 2-262) protein (His tag)Crystallography grade PMM1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Phosphomannomutase 1 (PMM1) protein (Myc-DYKDDDDK Tag)PMM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 1 mM DTT, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PMM1 (Phosphomannomutase 1 (PMM1))
- Alternative Name
- Phosphomannomutase 1/PMM1 (PMM1 Products)
- Synonyms
- Sec53 Protein, C77612 Protein, phosphomannomutase 1 Protein, phosphomannomutase 1 L homeolog Protein, PMM1 Protein, pmm1 Protein, Pmm1 Protein, pmm1.L Protein
- Sub Type
- Fusionprotein
- Background
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Phosphomannomutase 1 (PMM1) blongs to the eukaryotic PMM family. Phosphomannomutase 1 can catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose which required for a number of critical mannosyl transfer reactions. PMM1 is highly expressed in liver, heart, brain, and pancreas, but lower expression in skeletal muscle. In addition, PMM1 may be responsible for the degradation of glucose-1,6 bisphosphate in ischemic brain.
Alternative Names: Phosphomannomutase 1, PMM 1, PMMH-22, PMM1, PMMH22 - Molecular Weight
- 30.8 kDa
- UniProt
- Q92871
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