PPM1A Protein (AA 2-382) (His tag)

Catalog No. ABIN1097373

Product Details

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Protein Type: Recombinant

Protein Characteristics: AA 2-382

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PPM1A protein is labelled with His tag.

Purpose: Recombinant Human Protein Phosphatase 1A/PPM1A (C-6His)

Sequence: MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ESWSFFAVYD GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVHFF TQDHKPSNPL EKERIQNAGG SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSPE AVKKEAELDK YLECRVEEII KKQGEGVPDL VHVMRTLASE NIPSLPPGGE LASKRNVIEA VYNRLNPYKN DDTDSTSTDD MWLEHHHHHH

Characteristics: Recombinant Human Protein Phosphatase 1A/PPM1A is produced with our E. coli expression system. The target protein is expressed with sequence (Gly2-Trp382) of Human PPM1A.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Alternative Name: Protein Phosphatase 1A/PPM1A (PPM1A Products)

Synonyms: PP2C-ALPHA Protein, PP2CA Protein, PP2Calpha Protein, pp2ca1 Protein, ppp1r13b Protein, si:ch211-253p18.1 Protein, wu:fd42g12 Protein, zgc:153820 Protein, 2310003C21Rik Protein, 2900017D14Rik Protein, AI427932 Protein, AU017636 Protein, MMPa-2 Protein, MPPa-1 Protein, Pp2c1 Protein, pp2c-alpha Protein, pp2ca Protein, pp2calpha Protein, pp2ca2 Protein, protein phosphatase, Mg2+/Mn2+ dependent 1A Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1Ab Protein, protein phosphatase 1A, magnesium dependent, alpha isoform Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1A Protein, protein phosphatase, Mg2+/Mn2+ dependent 1A L homeolog Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1Aa Protein, PPM1A Protein, ppm1ab Protein, Ppm1a Protein, ppm1a.L Protein, ppm1aa Protein

Background: Protein Phosphatase 1A (PPM1A) is a member of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1A has a broad specificity. PPM1A negatively regulates the activities of MAP kinases and MAP kinase kinases. Also, it negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. In addition, PPM1A can dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control.
Alternative Names: Protein Phosphatase 1A, Protein Phosphatase 2C Isoform Alpha, PP2C-Alpha, Protein Phosphatase IA, PPM1A, PPPM1A

Molecular Weight: 43.5 kDa

UniProt: P35813