PFDN2 Protein (AA 1-154) (His tag)
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- Target See all PFDN2 Proteins
- PFDN2 (Prefoldin Subunit 2 (PFDN2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-154
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PFDN2 protein is labelled with His tag.
- Purpose
- Recombinant Human Prefoldin Subunit 2/PFDN2 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAENSGRAGK SSGSGAGKGA VSAEQVIAGF NRLRQEQRGL ASKAAELEME LNEHSLVIDT LKEVDETRKC YRMVGGVLVE RTVKEVLPAL ENNKEQIQKI IETLTQQLQA KGKELNEFRE KHNIRLMGED EKPAAKENSE GAGAKASSAG VLVS
- Characteristics
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Recombinant Human Prefoldin Subunit 2/PFDN2 (N-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PFDN2 Protein
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Prefoldin Subunit 2 (PFDN2) (AA 1-154) protein (GST tag)
PFDN2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Prefoldin Subunit 2 (PFDN2) (AA 1-154) protein (Strep Tag)Crystallography grade PFDN2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Prefoldin Subunit 2 (PFDN2) protein (Myc-DYKDDDDK Tag)PFDN2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 50 mM NaCl, 1 mM DTT, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PFDN2 (Prefoldin Subunit 2 (PFDN2))
- Alternative Name
- Prefoldin Subunit 2/PFDN2 (PFDN2 Products)
- Synonyms
- im:6895552 Protein, wu:fb79a01 Protein, zgc:136473 Protein, ESTM27 Protein, W48336 Protein, PFD2 Protein, prefoldin subunit 2 Protein, prefoldin 2 Protein, pfdn2 Protein, EHI_113900 Protein, AOR_1_1714154 Protein, Pfdn2 Protein, PFDN2 Protein
- Background
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Recombinant Human Prefoldin Subunit 2/PFDN2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser143) of Human PFDN2 fused with a His tag at the N-terminus.
Prefoldin Subunit 2 (PFDN2) belongs to the Prefoldin β subunit family. The PFDN2 protein is one of six subunits of Prefoldin that act as a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides allowing them to fold correctly. PFDN2 binds specifically to Cytosolic Chaperonin (c-CPN) and transfers target proteins to it. PFDN2 also binds to a nascent polypeptide chain and promotes folding in settings where there are many competing pathways for non-native proteins. - Molecular Weight
- 18.8 kDa
- UniProt
- Q9UHV9
- Pathways
- Unfolded Protein Response
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