PFDN4 Protein (AA 1-134) (His tag)

Catalog No. ABIN1097400

Product Details

Target: PFDN4 (Prefoldin Subunit 4 (PFDN4))

Protein Type: Recombinant

Protein Characteristics: AA 1-134

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PFDN4 protein is labelled with His tag.

Purpose: Recombinant Human Prefoldin Subunit 4/PFDN4 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAATMKKAAA EDVNVTFEDQ QKINKFARNT SRITELKEEI EVKKKQLQNL EDACDDIMLA DDDCLMIPYQ IGDVFISHSQ EETQEMLEEA KKNLQEEIDA LESRVESIQR VLADLKVQLY AKFGSNINLE ADES

Characteristics: Recombinant Human Prefoldin Subunit 4/PFDN4 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser134) of Human PFDN4 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: PFDN4 (Prefoldin Subunit 4 (PFDN4))

Alternative Name: PFDN4 (PFDN4 Products)

Synonyms: DDBDRAFT_0204261 Protein, DDBDRAFT_0237717 Protein, DDB_0204261 Protein, DDB_0237717 Protein, pfd4 Protein, C1 Protein, PFD4 Protein, 0610025M10Rik Protein, 1810063B06Rik Protein, C-1 Protein, Pfd4 Protein, si:dkey-222e15.2 Protein, prefoldin subunit 4 Protein, prefoldin beta-like domain containing protein Protein, Prefoldin subunit 4 Protein, prefoldin 4 Protein, PFDN4 Protein, AFUA_1G15240 Protein, AOR_1_2080174 Protein, CMU_006340 Protein, pfdn4 Protein, pfd4 Protein, Pfdn4 Protein

Sub Type: Fusionprotein

Background: Prefoldin Subunit 4 (PFDN4) is a heterohexameric chaperone protein that belongs to the prefoldin subunit beta family. The complex of PFDN4, consisting of two PFD-alpha type and four PFD-beta type subunits, forms a double beta barrel assembly with six protruding coiled-coils. PFDN4 binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly.
Alternative Names: Prefoldin Subunit 4, Protein C-1, PFDN4, PFD4

Molecular Weight: 17.48 kDa

UniProt: Q9NQP4

Pathways: Unfolded Protein Response