RAGE Protein (AA 23-344) (His tag)

Catalog No. ABIN1097457

Product Details

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Protein Type: Recombinant

Protein Characteristics: AA 23-344

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This RAGE protein is labelled with His tag.

Purpose: Recombinant Human AGER/RAGE (C-6His)

Sequence: AQNITARIGE PLVLKCKGAP KKPPQRLEWK LNTGRTEAWK VLSPQGGGPW DSVARVLPNG SLFLPAVGIQ DEGIFRCQAM NRNGKETKSN YRVRVYQIPG KPEIVDSASE LTAGVPNKVG TCVSEGSYPA GTLSWHLDGK PLVPNEKGVS VKEQTRRHPE TGLFTLQSEL MVTPARGGDP RPTFSCSFSP GLPRHRALRT APIQPRVWEP VPLEEVQLVV EPEGGAVAPG GTVTLTCEVP AQPSPQIHWM KDGVPLPLPP SPVLILPEIG PQDQGTYSCV ATHSSHGPQE SRAVSISIIE PGEEGPTAGS VGGSGLGTLA LAVDHHHHHH

Characteristics: Recombinant Human Advanced Glycosylation End Product-Specific Receptor/AGER produced by transfected human cells is a secreted protein with sequence(Ala23-Ala344) of Human AGER fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: sRage (AGER Products)

Synonyms: RAGE Protein, AGER Protein, advanced glycosylation end-product specific receptor Protein, advanced glycosylation end product-specific receptor Protein, MAPK/MAK/MRK overlapping kinase Protein, AGER Protein, Ager Protein, LOC719012 Protein

Sub Type: Fusionprotein

Background: Advanced Glycosylation End Product-Specific Receptor (AGER) belongs to the immunoglobulin superfamily of cell surface molecules. It lies within the major histocompatibility complex (MHC) class III region on chromosome 6. Besides AGEs, AGER is also able to bind other ligands which is thought to result in pro-inflammatory gene activation. It is known that AGER serve as a mediator of both acute and chronic vascular inflammation in certain conditions such as atherosclerosis and in particular as a complication of diabetes. Furthermore, it plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes.
Alternative Names: Advanced Glycosylation End Product-Specific Receptor, Receptor for Advanced Glycosylation End Products, AGER, RAGE

Molecular Weight: 35.2 kDa

UniProt: Q15109

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins