RNASE1 Protein (AA 29-156) (His tag)
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- Target See all RNASE1 Proteins
- RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-156
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This RNASE1 protein is labelled with His tag.
- Purpose
- Recombinant Human Ribonuclease Pancreatic/RNASE1 (C-6His)
- Sequence
- KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDSTVD HHHHHH
- Characteristics
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Recombinant Human Ribonuclease Pancreatic/RNASE1 (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product RNASE1 Protein
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Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 29-128) protein (GST tag)
RNASE1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 1-156) protein (GST tag)RNASE1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 29-156) protein (His tag)Crystallography grade RNASE1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 26-149) protein (His tag)Crystallography grade RNASE1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 10 % Glycerol, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
- Alternative Name
- Ribonuclease Pancreatic/RNASE1 (RNASE1 Products)
- Synonyms
- RNASE1 Protein, RNS1 Protein, ATRNS1 Protein, RIBONUCLEASE 1 Protein, T17M13.16 Protein, T17M13_16 Protein, ribonuclease 1 Protein, RIB1 Protein, AI574248 Protein, Rib-1 Protein, Rib1 Protein, SRN Protein, ribonuclease A family member 1, pancreatic Protein, ribonuclease pancreatic-like Protein, ribonuclease A family member k6 Protein, Ribonuclease 1 Protein, ribonuclease 1 Protein, ribonuclease, RNase A family, 1 (pancreatic) Protein, ribonuclease pancreatic Protein, RNASE1 Protein, LOC475395 Protein, RNASE6 Protein, Rnase1 Protein, RNS1 Protein, LOC101113761 Protein
- Background
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Recombinant Human Ribonuclease Pancreatic/RNASE1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Lys29-Thr156) of Human RNASE1 fused with a 6His tag at the C-terminus.
Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNASE1 is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. RNASE1 prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNASE1 acts on single stranded and double stranded RNA. - Molecular Weight
- 15.6 kDa
- UniProt
- P07998
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