SCLY Protein (AA 1-445) (His tag)

Catalog No. ABIN1097475

Product Details

Target: SCLY (Selenocysteine Lyase (SCLY))

Protein Type: Recombinant

Protein Characteristics: AA 1-445

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SCLY protein is labelled with His tag.

Purpose: Recombinant Human Selenocysteine Lyase/SCLY (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MEAAVAPGRD APAPAASQPS GCGKHNSPER KVYMDYNATT PLEPEVIQAM TKAMWEAWGN PSSPYSAGRK AKDIINAARE SLAKMIGGKP QDIIFTSGGT ESNNLVIHSV VKHFHANQTS KGHTGGHHSP VKGAKPHFIT SSVEHDSIRL PLEHLVEEQV AAVTFVPVSK VSGQTEVDDI LAAVRPTTRL VTIMLANNET GIVMPVPEIS QRIKALNQER VAAGLPPILV HTDAAQALGK QRVDVEDLGV DFLTIVGHKF YGPRIGALYI RGLGEFTPLY PMLFGGGQER NFRPGTENTP MIAGLGKAAE LVTQNCEAYE AHMRDVRDYL EERLEAEFGQ KRIHLNSQFP GTQRLPNTCN FSIRGPRLQG HVVLAQCRVL MASVGAACHS DHGDQPSPVL LSYGVPFDVA RNALRLSVGR STTRAEVDLV VQDLKQAVAQ LEDQA

Characteristics: Recombinant Human Selenocysteine Lyase/SCLY is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala445) of Human SCLY fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: SCLY (Selenocysteine Lyase (SCLY))

Alternative Name: SCL (SCLY Products)

Synonyms: SCL Protein, hSCL Protein, 9830169H08 Protein, A930015N15Rik Protein, Scly1 Protein, Scly2 Protein, zgc:171514 Protein, selenocysteine lyase Protein, selenocysteine lyase L homeolog Protein, Selenocysteine lyase Protein, SCLY Protein, Scly Protein, scly.L Protein, csdB Protein, EUBELI_20129 Protein, Deima_1599 Protein, scly Protein

Sub Type: Fusionprotein

Background: Selenocysteine Lyase belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Selenocysteine Lyase exists as a homodimer in the cytosol. In the brain, Selenocysteine Lyase is as an enzyme that putatively salvages Sec and recycles the selenium for selenoprotein translation. Selenocysteine Lyase catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium. Selenocysteine Lyase can be up-regulated In acute glomerulonephritis, it can also be regulated by JUN/AP-1.
Alternative Names: Selenocysteine Lyase, hSCL, SCLY, SCL

Molecular Weight: 50.34 kDa

UniProt: Q96I15

Pathways: Stem Cell Maintenance