TES Protein (AA 1-421) (His tag)

Catalog No. ABIN1097622

Product Details

Target: TES (Testis Derived Transcript (3 LIM Domains) (TES))

Protein Type: Recombinant

Protein Characteristics: AA 1-421

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TES protein is labelled with His tag.

Purpose: Recombinant Human Testin/TES (C-6His)

Sequence: MDLENKVKKM GLGHEQGFGA PCLKCKEKCE GFELHFWRKI CRNCKCGQEE HDVLLSNEED RKVGKLFEDT KYTTLIAKLK SDGIPMYKRN VMILTNPVAA KKNVSINTVT YEWAPPVQNQ ALARQYMQML PKEKQPVAGS EGAQYRKKQL AKQLPAHDQD PSKCHELSPR EVKEMEQFVK KYKSEALGVG DVKLPCEMDA QGPKQMNIPG GDRSTPAAVG AMEDKSAEHK RTQYSCYCCK LSMKEGDPAI YAERAGYDKL WHPACFVCST CHELLVDMIY FWKNEKLYCG RHYCDSEKPR CAGCDELIFS NEYTQAENQN WHLKHFCCFD CDSILAGEIY VMVNDKPVCK PCYVKNHAVV CQGCHNAIDP EVQRVTYNNF SWHASTECFL CSCCSKCLIG QKFMPVEGMV FCSVECKKRM SLEHHHHHH

Characteristics: Recombinant Human Testin/TES is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser421) of Human TES fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.2.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: TES (Testis Derived Transcript (3 LIM Domains) (TES))

Alternative Name: Testin (TES Products)

Synonyms: TESS Protein, TESS-2 Protein, tess Protein, tess-2 Protein, zgc:92091 Protein, xtes Protein, TES Protein, DKFZp469E2320 Protein, D6Ertd352e Protein, Tes1 Protein, Tes2 Protein, testin2 Protein, Testin Protein, testin LIM domain protein Protein, testin LIM domain protein L homeolog Protein, testis derived transcript (3 LIM domains) Protein, testis derived transcript Protein, TES Protein, tes.L Protein, tes Protein, Tes Protein

Sub Type: Fusionprotein

Background: Testin belongs to the prickle/espinas/testin family. Testin contains three LIM zinc-binding domains and one PET domain, TES as a scaffold protein that may have a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. In addition, TES can also as a tumor suppressor, inhibits tumor cell growth, regulate the cell proliferation.TES interacts with many cytoskeletal proteins, such as Zyxin, Mena, Actin, Talin and VASP. The ability of TES to associate with alpha-actin, Zyxin and paxillin is dependent on the conformational state of the molecule.
Alternative Names: Testin, TESS, TES

Molecular Weight: 49.1 kDa

UniProt: Q9UGI8