Thimet Oligopeptidase 1 Protein (THOP1) (AA 2-689) (His tag)

Catalog No. ABIN1097631

Product Details

Target: Thimet Oligopeptidase 1 (THOP1)

Protein Type: Recombinant

Protein Characteristics: AA 2-689

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Thimet Oligopeptidase 1 protein is labelled with His tag.

Purpose: Recombinant Human Thimet Oligopeptidase/THOP1 (C-6His)

Sequence: MKPPAACAGD MADAASPCSV VNDLRWDLSA QQIEERTREL IEQTKRVYDQ VGTQEFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP SKDIRTASTE ADKKLSEFDV EMSMREDVYQ RIVWLQEKVQ KDSLRPEAAR YLERLIKLGR RNGLHLPRET QENIKRIKKK LSLLCIDFNK NLNEDTTFLP FTLQELGGLP EDFLNSLEKM EDGKLKVTLK YPHYFPLLKK CHVPETRRKV EEAFNCRCKE ENCAILKELV TLRAQKSRLL GFHTHADYVL EMNMAKTSQT VATFLDELAQ KLKPLGEQER AVILELKRAE CERRGLPFDG RIRAWDMRYY MNQVEETRYC VDQNLLKEYF PVQVVTHGLL GIYQELLGLA FHHEEGASAW HEDVRLYTAR DAASGEVVGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQIAIAAMVA NFTKPTADAP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEQEPLLR MSRHYRTGSA VPRELLEKLI ESRQANTGLF NLRQIVLAKV DQALHTQTDA DPAEEYARLC QEILGVPATP GTNMPATFGH LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLNSKVGMD YRSCILRPGG SEDASAMLRR FLGRDPKQDA FLLSKGLQVG GCEPEPQVCL EHHHHHH

Characteristics: Recombinant Human Thimet Oligopeptidase/THOP1 is produced by our E. coli expression system. The target protein is expressed with sequence (Lys2-Cys689) of Human THOP1.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, 10 % Glycerol, pH 7.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Thimet Oligopeptidase 1 (THOP1)

Alternative Name: Thimet Oligopeptidase/THOP1 (THOP1 Products)

Synonyms: EP24.15 Protein, MEPD_HUMAN Protein, MP78 Protein, TOP Protein, AI131655 Protein, AI327041 Protein, THOP1 Protein, thop1 Protein, MGC147297 Protein, LOC397806 Protein, mepd Protein, thimet oligopeptidase 1 Protein, thimet oligopeptidase 1 L homeolog Protein, thimet oligopeptidase Protein, peptidase Protein, Thimet oligopeptidase Protein, THOP1 Protein, Thop1 Protein, thop1 Protein, thop1.L Protein, Tc00.1047053506411.10 Protein, Tc00.1047053511237.10 Protein, Noca_4613 Protein, ANI_1_1400064 Protein, MBOO_RS10705 Protein, AOR_1_880174 Protein, AnaeK_0425 Protein, MPAL_RS03920 Protein, Hoch_5061 Protein, mepd Protein

Background: Thimet Oligopeptidase (THOP1) belongs to the peptidase M3 family which includes neurolysin and mitochondrial intermediate peptidase. THOP1 is located in Cytoplasm. THOP1 is widely expressed in human tissues and can detected in different subcellular locations. THOP1 is preferential cleavage for bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues. THOP1 is involved in the metabolism of neuropeptides under 20 amino acid residues and degradation of cytoplasmic peptide. In addition, THOP1 also can degrade the beta-amyloid precursor protein and generate amyloidogenic fragments.
Alternative Names: Thimet Oligopeptidase, Endopeptidase 24.15, MP78, THOP1

Molecular Weight: 80 kDa

UniProt: P52888