TOLLIP Protein (AA 2-274) (His tag)

Catalog No. ABIN1097679

Product Details

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Protein Type: Recombinant

Protein Characteristics: AA 2-274

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TOLLIP protein is labelled with His tag.

Purpose: Recombinant Human Toll-Interacting Protein/TOLLIP (C-6His)

Sequence: ATTVSTQRGP VYIGELPQDF LRITPTQQQR QVQLDAQAAQ QLQYGGAVGT VGRLNITVVQ AKLAKNYGMT RMDPYCRLRL GYAVYETPTA HNGAKNPRWN KVIHCTVPPG VDSFYLEIFD ERAFSMDDRI AWTHITIPES LRQGKVEDKW YSLSGRQGDD KEGMINLVMS YALLPAAMVM PPQPVVLMPT VYQQGVGYVP ITGMPAVCSP GMVPVALPPA AVNAQPRCSE EDLKAIQDMF PNMDQEVIRS VLEAQRGNKD AAINSLLQMG EEPVEHHHHH H

Characteristics: Recombinant Human Toll-Interacting Protein is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Pro274) of Human TOLLIP fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5 mM EDTA, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Alternative Name: Tollip (TOLLIP Products)

Synonyms: IL-1RAcPIP Protein, 4930403G24Rik Protein, 4931428G15Rik Protein, fk22a02 Protein, im:7145391 Protein, wu:fk22a02 Protein, zgc:76985 Protein, TOLLIP Protein, GB17961 Protein, tollip Protein, tollip1 Protein, tollipa Protein, TOLIP Protein, toll interacting protein Protein, toll-interacting protein Protein, toll-interacting protein-like Protein, toll interacting protein L homeolog Protein, toll interacting protein S homeolog Protein, Toll-interleukine I receptor interacting protein I Protein, TOLLIP Protein, Tollip Protein, tollip Protein, LOC552034 Protein, LOC100169858 Protein, tollip.L Protein, tollip.S Protein, LOC100136122 Protein

Sub Type: Fusionprotein

Background: Toll-Interacting Protein (TOLLIP) is a member of the tollip family. TOLLIP localizes to the cytoplasm. It contains one C2 domain and one CUE domain. TOLLIP is an inhibitory adaptor protein for Toll-like receptors (TLR). The Toll-like receptors pathway is a part of the immune system that recognize structurally conserved molecular patterns of microbial pathogens, resulting in an inflammatory immune response. TOLLIP constitutes a complex with Tom1 to regulate endosomal transferring of ubiquitinated proteins. TOLLIP can negative regulate Toll-like receptors signaling, which may limit the production of proinflammatory mediators during the process of inflammation and infection.
Alternative Names: Toll-Interacting Protein, TOLLIP

Molecular Weight: 31.3 kDa

UniProt: Q9H0E2