UBE2D4 Protein (AA 1-147) (GST tag)

Catalog No. ABIN1097727

Product Details

Target: UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))

Protein Type: Recombinant

Protein Characteristics: AA 1-147

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2D4 protein is labelled with GST tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 D4/UBE2D4 (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 D4/UBE2D4 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Met147) of Human UBE2D4 fused with a GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))

Alternative Name: Ubiquitin-Conjugating Enzyme E2 D4/UBE2D4 (UBE2D4 Products)

Synonyms: HBUCE1 Protein, UBE2D4 Protein, RGD69425 Protein, Ube2d2 Protein, Ube2d2b Protein, zgc:162263 Protein, ube2d3.2 Protein, EG546638 Protein, Gm5959 Protein, ubiquitin conjugating enzyme E2 D4 (putative) Protein, ubiquitin-conjugating enzyme E2D 4 (putative) Protein, ubiquitin-conjugating enzyme E2 D4 Protein, ubiquitin conjugating enzyme E2D 4 (putative) L homeolog Protein, ubiquitin-conjugating enzyme E2 D2 pseudogene Protein, ubiquitin-conjugating enzyme E2D 4 Protein, UBE2D4 Protein, Ube2d4 Protein, EDI_085450 Protein, EDI_185830 Protein, ube2d4 Protein, ube2d4.L Protein, LOC100354069 Protein

Sub Type: Fusionprotein

Background: Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2D4 has been proposed to participate in Ubl conjugation pathway. UBE2D4 takes part in post-translational protein modification, protein K6-linked ubiquitination, protein K11-linked ubiquitination, protein K27-linked ubiquitination, protein K29-linked ubiquitination, protein K48-linked ubiquitination, and protein K63-linked ubiquitination. UBE2D4 regulate of protein metabolic process. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 D4, HBUCE1, Ubiquitin Carrier Protein D4, Ubiquitin-Protein Ligase D4, UBE2D4, UBCH5D

Molecular Weight: 43.45 kDa

UniProt: Q9Y2X8