UBE2J2 Protein (AA 124-224) (GST tag)

Catalog No. ABIN1097745

Product Details

Target: UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))

Protein Type: Recombinant

Protein Characteristics: AA 124-224

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2J2 protein is labelled with GST tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 J2/UBE2J2 (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFH MEKGPTLGSI ETSDFTKRQL AVQSLAFNLK DKVFCELFPE VVEEIKQKQK AQDELSSRPQ TLPLPDVVPD GETHLVQNGI QLLNGHAPGA VPNLAGLQQA NR

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 J2/UBE2J2 is produced by our E. coli expression system. Target protein is expressed with sequence (Glu124-Arg224) of Human UBE2J2 fused with a GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.2.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))

Alternative Name: Ubiquitin-Conjugating Enzyme E2 J2/UBE2J2 (UBE2J2 Products)

Synonyms: NCUBE-2 Protein, NCUBE2 Protein, PRO2121 Protein, 1200007B18Rik Protein, 2400008G19Rik Protein, 5730472G04Rik Protein, AL022923 Protein, Ubc6 Protein, Ubc6p Protein, zgc:162164 Protein, ubiquitin conjugating enzyme E2 J2 Protein, ubiquitin-conjugating enzyme E2J 2 Protein, ubiquitin-conjugating enzyme E2, J2 Protein, ubiquitin-conjugating enzyme E2 J2 Protein, ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) Protein, ubiquitin conjugating enzyme E2 J2 L homeolog Protein, UBE2J2 Protein, Ube2j2 Protein, LOC5574000 Protein, CpipJ_CPIJ008956 Protein, ube2j2 Protein, uce2 Protein, ube2j2.L Protein, LOC100067387 Protein

Sub Type: Fusionprotein

Background: Ubiquitin-Conjugating Enzyme E2 J2 (UBE2J2) belongs to the ubiquitin-conjugating enzyme family. UBE2J2 is involved in the ubiquitiantion. UBE2J2 located in the membrane of the endoplasmic reticulum, catalyzes the covalent attachment of ubiquitin to other proteins. UBE2J2 may play a important role in the selective degradation of misfolded membrane protein from the endoplasmic reticulum.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 J2, Non-Canonical Ubiquitin-Conjugating Enzyme 2, NCUBE-2, UBE2J2, NCUBE2

Molecular Weight: 37.96 kDa

UniProt: Q8N2K1