NDUFA5 Protein (His tag)
-
- Target See all NDUFA5 Proteins
- NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NDUFA5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product NDUFA5 Protein
-
-
- Comment
-
Synonyms: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, B13, CI-13kB, CI-13KD-B, NUFM, UQOR13
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
-
- Target
- NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))
- Alternative Name
- NDUFA5 (NDUFA5 Products)
- Synonyms
- NADHUO Protein, B13 Protein, CI-13KD-B Protein, CI-13kB Protein, NUFM Protein, UQOR13 Protein, 2900002J19Rik Protein, NADH:ubiquinone oxidoreductase subunit A5 Protein, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 Protein, Ndufa5 Protein, NDUFA5 Protein
- Background
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, also known as NDUFA5, belongs to the complex I NDUFA5 subunit family. The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. Recombinant human NDUFA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
- Molecular Weight
- 15.8 kDa (139aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_004991
-