IFI30 Protein (His tag)
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- Target See all IFI30 Proteins
- IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IFI30 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product IFI30 Protein
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Interferon, gamma-Inducible Protein 30 (IFI30) (AA 58-232) protein (His tag)
IFI30 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interferon, gamma-Inducible Protein 30 (IFI30) (AA 1-250) protein (GST tag)IFI30 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interferon, gamma-Inducible Protein 30 (IFI30) (AA 58-232) protein (His tag)Crystallography grade IFI30 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interferon, gamma-Inducible Protein 30 (IFI30) (AA 55-230) protein (His tag)Crystallography grade IFI30 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interferon, gamma-Inducible Protein 30 (IFI30) protein (Myc-DYKDDDDK Tag)IFI30 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Gamma-interferon-inducible lysosomal thiol reductase, GILT, IFI-30, IP30
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))
- Alternative Name
- IFI30 (IFI30 Products)
- Synonyms
- GILT Protein, wu:fb12b04 Protein, zgc:101752 Protein, IP30 Protein, IFI-30 Protein, interferon, gamma-inducible protein 30 Protein, IFI30, lysosomal thiol reductase Protein, interferon gamma inducible protein 30 Protein, ifi30 Protein, IFI30 Protein, LOC100304719 Protein, Ifi30 Protein
- Background
- IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- Molecular Weight
- 22.5 kDa (199aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_006323
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