ADH5 Protein (His tag)

Catalog No. ABIN1098240

Product Details

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ADH5 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Purity: > 90 % by SDS - PAGE

Application Details

Comment:

Synonyms: Alcohol dehydrogenase class-3, ADHX, FDH

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH7.5) containing 20% glycerol 0.1 M Nacl, 1 MM DTT

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Alternative Name: ADH5 (ADH5 Products)

Synonyms: ADH2 Protein, ADH-3 Protein, ADHX Protein, FALDH Protein, FDH Protein, GSH-FDH Protein, GSNOR Protein, ADH1C Protein, ADH3 Protein, wu:fb60b11 Protein, Adh-5 Protein, Adh3 Protein, adh-3 Protein, adh3 Protein, adh5 Protein, adhx Protein, fdh Protein, gsnor Protein, ADH4 Protein, ADH5 Protein, alcohol dehydrogenase 1B (class I), beta polypeptide Protein, alcohol dehydrogenase 5 (class III), chi polypeptide Protein, alcohol dehydrogenase 5 Protein, alcohol dehydrogenase 5 (class III), chi polypeptide L homeolog Protein, alcohol dehydrogenase class-3 Protein, ADH1B Protein, ADH5 Protein, adh5 Protein, Adh5 Protein, adh5.L Protein, LOC101112223 Protein

Background: ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. Recombinant human ADH5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 42.3 kDa (398aa), confirmed by MALDI-TOF

NCBI Accession: NP_000662