UBE2F Protein (His tag)
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- Target See all UBE2F Proteins
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBE2F protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product UBE2F Protein
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Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) protein (GST tag)
UBE2F Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) protein (Strep Tag)Crystallography grade UBE2F Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) protein (Strep Tag)Crystallography grade UBE2F Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Ubiquitin-Conjugating Enzyme E2F (UBE2F) protein (Myc-DYKDDDDK Tag)UBE2F Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: NEDD8-conjugating enzyme UBE2F, NCE2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer, pH 8.0, 30% glycerol, 1 MM DTT, 200 mM NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- Alternative Name
- UBE2F (UBE2F Products)
- Synonyms
- NCE2 Protein, 2510010F15Rik Protein, AI851109 Protein, RGD1307608 Protein, zgc:77005 Protein, ubiquitin conjugating enzyme E2 F (putative) Protein, NEDD8-conjugating enzyme UBE2F Protein, ubiquitin-conjugating enzyme E2F (putative) Protein, ubiquitin conjugating enzyme E2F (putative) S homeolog Protein, UBE2F Protein, Tsp_01616 Protein, ube2f Protein, ube2f.S Protein, Ube2f Protein
- Background
- UBE2F, also Known as Ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and UBE2F subfamily. Ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human UBE2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 23.7kDa (209aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_542409
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