GCLM Protein (His tag)
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- Target See all GCLM Proteins
- GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GCLM protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product GCLM Protein
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Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (GST tag)
GCLM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (Strep Tag)Crystallography grade GCLM Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (Strep Tag)Crystallography grade GCLM Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) protein (Myc-DYKDDDDK Tag)GCLM Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Glutamate-cysteine ligase, modifier subunit, GLCLR
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15 M NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))
- Alternative Name
- GCLM (GCLM Products)
- Synonyms
- Glclr Protein, GLCLR Protein, id:ibd3182 Protein, wu:fi24c07 Protein, zgc:55903 Protein, AI649393 Protein, Gcmc Protein, glutamate-cysteine ligase regulatory subunit Protein, glutamate cysteine ligase, modifier subunit Protein, glutamate-cysteine ligase modifier subunit Protein, glutamate-cysteine ligase, modifier subunit Protein, glutamate-cysteine ligase, modifier subunit L homeolog Protein, PTRG_09814 Protein, Gclm Protein, GCLM Protein, gclm Protein, gclm.L Protein
- Background
- Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Recombinant human GCLM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 33.3kDa(298aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_002052
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