CLTB Protein (His tag)
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- Target See all CLTB Proteins
- CLTB (Clathrin, Light Chain B (CLTB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CLTB protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product CLTB Protein
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- Comment
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Synonyms: clathrin, light chain B, LCB
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol, 0.2 MM PMSF, 100 mM NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- CLTB (Clathrin, Light Chain B (CLTB))
- Alternative Name
- CLTB (CLTB Products)
- Synonyms
- clcb Protein, LCB Protein, CLTLB Protein, 2310046E19Rik Protein, clathrin, light chain B L homeolog Protein, clathrin light chain B Protein, Clathrin light chain B Protein, clathrin, light chain B Protein, clathrin, light polypeptide (Lcb) Protein, cltb.L Protein, CLTB Protein, clcb Protein, Cltb Protein
- Background
- CLTB (Clathrin light chain B) belongs to the clathrin light chain family. Clathrin is a protein that plays a major role in the formation of coated vesicles. It forms a triskelion shape composed of three clathrin heavy chains and three light chains. When the triskelia interact they form a polyhedral lattice that surrounds the vesicle. Coat-proteins, like clathrin, are used to build small vesicles in order to safely transport molecules between cells. Clathrin-mediated endocytosis (CME) regulates many cellular physiological processes such as the internalization of growth factors and receptors, entry of pathogens, and synaptic transmission. Recombinant human CLTB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 25.6kDa (234aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
- NCBI Accession
- NP_001825
- Pathways
- Synaptic Membrane
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