NHEJ1 Protein (His tag)
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- Target See all NHEJ1 Proteins
- NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NHEJ1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product NHEJ1 Protein
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Nonhomologous End-Joining Factor 1 (NHEJ1) (AA 1-224) protein (His tag)
NHEJ1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nonhomologous End-Joining Factor 1 (NHEJ1) (AA 1-299) protein (GST tag)NHEJ1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nonhomologous End-Joining Factor 1 (NHEJ1) (AA 1-295) protein (Strep Tag)Crystallography grade NHEJ1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Nonhomologous End-Joining Factor 1 (NHEJ1) (AA 1-299) protein (Strep Tag)Crystallography grade NHEJ1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Nonhomologous End-Joining Factor 1 (NHEJ1) protein (Myc-DYKDDDDK Tag)NHEJ1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Non-homologous end-joining factor 1, XLF
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 20% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))
- Alternative Name
- NHEJ1 (NHEJ1 Products)
- Synonyms
- NHEJ1 Protein, zgc:85657 Protein, XLF Protein, 1700029B21Rik Protein, cernunnos Protein, non-homologous end joining factor 1 Protein, non-homologous end joining factor 1 S homeolog Protein, solute carrier family 23 member 3 Protein, nonhomologous end-joining factor 1 Protein, NHEJ1 Protein, nhej1.S Protein, nhej1 Protein, SLC23A3 Protein, Nhej1 Protein
- Background
- Non-homologous end-joining factor 1, also known as NHEJ1, belongs to the XLF family. NHEJ1 was originally discovered as the protein mutated in five patients with growth retardation, microcephaly, and immunodeficiency. The protein is required for the non-homologous end joining (NHEJ) pathway of DNA repair. Patients with NHEJ1 mutations also have immunodeficiency due to a defect in V(D)J recombination, which utilizes NHEJ to promote immune system diversity. XLF interacts with DNA ligase IV and XRCC4 and is thought to be involved in the ligation step of NHEJ. Recombinant human NHEJ1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 27.8 kDa (247aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_079058
- Pathways
- DNA Damage Repair
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