UBE2E1 Protein (His tag)

Catalog No. ABIN1098380

Product Details

Target: UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2E1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Purity: > 90 % by SDS - PAGE

Application Details

Comment:

Synonyms: Ubiquitin-conjugating enzyme E2 E1 isoform 1, UBCH6

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 20% glycerol

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))

Alternative Name: UBE2E1 (UBE2E1 Products)

Synonyms: ubch6 Protein, UBCH6 Protein, UbcM3 Protein, Ubce5 Protein, ubcM2 Protein, ubiquitin conjugating enzyme E2 E1 Protein, ubiquitin-conjugating enzyme E2 E1 Protein, ubiquitin-conjugating enzyme E2E 1 Protein, ubiquitin conjugating enzyme E2 E1 L homeolog Protein, ube2e1 Protein, UBE2E1 Protein, LOC100354960 Protein, LOC100362535 Protein, Ube2e1 Protein, ube2e1.L Protein

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2E1 is a member of the E2 ubiquitin-conjugating enzyme family. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Recombinant human UBE2E1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 23.8 kDa (216aa), confirmed by MALDI-TOF

NCBI Accession: NP_003332

Pathways: Ubiquitin Proteasome Pathway