POLR3H Protein (His tag)
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- Target See all POLR3H Proteins
- POLR3H (Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLR3H protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product POLR3H Protein
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Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H) (AA 1-204) protein (His tag)
POLR3H Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H) (AA 1-204) protein (GST tag)POLR3H Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H) (AA 1-204) protein (Strep Tag)Crystallography grade POLR3H Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)POLR3H Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: DNA-directed RNA polymerase III subunit RPC8, RPC22.9, RPC8
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- POLR3H (Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H))
- Alternative Name
- POLR3H (POLR3H Products)
- Synonyms
- 5031409G22Rik Protein, RPC8 Protein, RPC22.9 Protein, polymerase (RNA) III (DNA directed) polypeptide H Protein, RNA polymerase III subunit H Protein, Polr3h Protein, POLR3H Protein
- Background
- POLR3H, also as known as A1665 and RPC8, belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. POLR3H acts as nuclear and cytosolic DNA sensor involved in innate immune response. Recombinant human POLR3H protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 25.3kDa (227aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_612211
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