NCF4 Protein (His tag)
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- Target See all NCF4 Proteins
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NCF4 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product NCF4 Protein
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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-339) protein (His tag)
NCF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-339) protein (GST tag)NCF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-100) protein (GST tag)NCF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-339) protein (Strep Tag)Crystallography grade NCF4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NCF4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Neutrophil cytosol factor 4, NCF, P40PHOX, SH3PXD4
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- Alternative Name
- NCF4 (NCF4 Products)
- Synonyms
- p40phox Protein, AI451400 Protein, NCF Protein, P40PHOX Protein, SH3PXD4 Protein, neutrophil cytosolic factor 4 Protein, ncf4 Protein, Ncf4 Protein, NCF4 Protein
- Background
- NCF4 is a cytosolic regulatory of the superoxide-producing phagocyte NADPH-oxidase, amulticomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integratedcatalytic core of the enzyme system. Recombinant human NCF4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 41.1 kDa (359aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_000622
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