TFB2M Protein (His tag)
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- Target See all TFB2M Proteins
- TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TFB2M protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product TFB2M Protein
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Transcription Factor B2, Mitochondrial (TFB2M) (AA 20-396) protein (His-IF2DI Tag)
TFB2M Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Transcription Factor B2, Mitochondrial (TFB2M) (AA 297-396) protein (GST tag)TFB2M Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Transcription Factor B2, Mitochondrial (TFB2M) (AA 1-396) protein (GST tag)TFB2M Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Transcription Factor B2, Mitochondrial (TFB2M) (AA 20-396) protein (His tag)Crystallography grade TFB2M Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Transcription Factor B2, Mitochondrial (TFB2M) (AA 44-396) protein (His tag)Crystallography grade TFB2M Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Transcription Factor B2, Mitochondrial (TFB2M) protein (Myc-DYKDDDDK Tag)TFB2M Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Dimethyladenosine transferase 2, mitochondrial, Hkp1, mtTFB2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))
- Alternative Name
- TFB2M (TFB2M Products)
- Synonyms
- CG3910 Protein, Dmel\\CG3910 Protein, TFB2 Protein, d-TF2BM Protein, d-mtTFB2 Protein, TFB2M Protein, sb:cb857 Protein, hkp1 Protein, Hkp1 Protein, mtTFB2 Protein, mitochondrial transcription factor B2 Protein, transcription factor B2, mitochondrial Protein, mtTFB2 Protein, TFB2M Protein, tfb2m Protein, Tfb2m Protein
- Background
- TFB2M is S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. This protein is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. TFB2M stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. Recombinant human TFB2M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 45.8 kDa (401aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_071761
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